Biology
Biology
10th Edition
ISBN: 9780321794260
Author: Audesirk, Teresa/ Audesirk
Publisher: Pearson College Div
Question
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Chapter 41, Problem 3AC
Summary Introduction

To determine:

Whether the techniques like artificial insemination of producing children present some ethical issues.

Introduction:

The couples, who cannot have children, opt for various techniques like artificial insemination of couple’s sperm in a surrogate mother, in vitro fertilization using couple’s egg or sperm and in vitro techniques utilizing the use of donor’s sperm or egg.

Summary Introduction

To determine:

The legal issues that might arise from the techniques like artificial insemination of producing children.

Introduction:

IVF has helped many infertile couples to have children. It has revolutionized the medical industry by making it possible for childless couples to have children. But still, it has some legal issues.

Summary Introduction

To determine:

The medical issues that might arise from the techniques like artificial insemination of producing children.

Introduction:

IVF is not only treatment for infertility but also a great technique for treatment of genetic problems. IVF, although has some medical risks along with the great benefits it provides.

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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?   2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?   3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1) What differences would you expect to see between the…
Overview of Transformation Protocol   -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes.   Questions: 1)What is the selectable marker in this experiment? How…
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