Biology
Biology
3rd Edition
ISBN: 9781259824913
Author: Hoefnagels, Mariëlle
Publisher: Mcgraw-hill Education,
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Chapter 4, Problem 8MCQ
Summary Introduction

Introduction:

Concentration gradient is the difference in the concentration of solutes present on the opposite side of the cell membrane. It is used to determine the molecules with no net charge present on them. It is affected by both “exergonic movement and spontaneous movement” of diffusion.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
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