The meaning of term systemic hazard should be explained. Concept Introduction: The term hazard explains the risk of damage and causes harm to the environment. The types of hazard are as follows: Physical hazard - These types of hazard are associated with the machine and vibrations. Biomechanical hazard − These types of hazard are associated with the repetitive motions. Chemical hazard − These types of hazard are associated with the hazardous chemicals and its usage.

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2. A moving bed adsorption column needs to be designed to separate hydrophobic proteins from a fermentation broth. The following adsorption equilibrium data was observed in preliminary isotherm studies. The resin used was activated carbon with a porosity of 0.2. The overall mass transfer coefficient was determined to be 10 h¹, and the ratio of volumetric flow rate of broth to resin is 10. Determine the diameter of the column if the column height is limited to 2.5 m (indoor operation) with a flow rate of 20 m³/h, influent concentration of 7 g/L, and effluent concentration of 0.1 g/L. qi (mg/kg) Ci (g/L) 0.1 4.7 7.5 0.25 10.6 0.5 15.0 1.0 23.7 2.5 33.5 5.0 41.1 7.5

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Chapter 4, Problem 4.12Q

Interpretation Introduction

Interpretation:

The meaning of term systemic hazard should be explained.

Concept Introduction:

The term hazard explains the risk of damage and causes harm to the environment.

The types of hazard are as follows:

Physical hazard - These types of hazard are associated with the machine and vibrations.

Biomechanical hazard − These types of hazard are associated with the repetitive motions.

Chemical hazard − These types of hazard are associated with the hazardous chemicals and its usage.

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2. A moving bed adsorption column needs to be designed to separate hydrophobic proteins from a fermentation broth. The following adsorption equilibrium data was observed in preliminary isotherm studies. The resin used was activated carbon with a porosity of 0.2. The overall mass transfer coefficient was determined to be 10 h¹, and the ratio of volumetric flow rate of broth to resin is 10. Determine the diameter of the column if the column height is limited to 2.5 m (indoor operation) with a flow rate of 20 m³/h, influent concentration of 7 g/L, and effluent concentration of 0.1 g/L. qi (mg/kg) Ci (g/L) 0.1 4.7 7.5 0.25 10.6 0.5 15.0 1.0 23.7 2.5 33.5 5.0 41.1 7.5

3. You are given a mixture of four proteins, whose properties are listed in the table below. Propose a process to purify each protein so that you end up with four solutions of pure protein. What resin would you use to bind the protein(s)? What changes to the buffer would you make to desorb the protein(s)? Contains an N-terminal His6-tag. Two 50 kDa subunits contain a non-heme Fe2+ in the active site. Protein Size (kDa) pl Specific Properties A 100 6.0 B 40 7.7 C 240 8.3 Ꭰ 225 5.5 Contains FAD redox center and an NADH binding domain. Composed of six 40-kDa subunits, each of which contains a [2Fe-2S] cluster. Composed of three subunits: 100 kDa structural subunit, 75 kDa subunit with a molybdopterin center, and 50 kDa subunit with FAD and an NADH binding domain.

b) Explain the key features of the Langmuir adsorption model - Drawing a diagram with empty and occupied sites. Show how new molecules would adsorb. drawing the diagram, showing free and empty sites, and their number (to use for next section) - Define the capacity and binding affinity parameters in terms of things shown on the diagram Defining the capacity and binding affinity parameters in terms of bound, free sites, and free molecules - Plot what would be a typical breakthrough curve and give an explanation approximately when breakthrough would occur plotting a typical sigmoidal breakthrough curve and saying it would certainly occur by the time capacity is used, but also could be much earlier if the affinity is low