Biology
4th Edition
ISBN: 9781259188121
Author: Peter Stiling, Robert Brooker, Linda Graham, Eric Widmaier
Publisher: McGraw-Hill Education
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Chapter 4, Problem 2TY
Summary Introduction
Introduction: Microscope refers to a tool which is used to visualize the structure and inner workings of a cell through magnification. Microscope takes an image which is referred to as a micrograph. A compound microscope refers to microscope having more than one lens and it was first constructed by Zacharias Jansen in 1595.
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Students have asked these similar questions
a. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not?
b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not?
c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob?
d. Why must you slowly turn the fine adjustment knob?
e. Is natural light or is artificial light used when using the microscope?
The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can
place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the
field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a
10X eyepiece, giving a total magnification of 100X.
0000
1mm
2mm
3mm
4mm
1mm
The resolving power of the microscope reveals:
a. Whether or not oil is needed to see a microorganism.
b. The maximum magnification possible with a particular objective. closely spaced objects to be seen clearly.
c. Two closely spaced objects to be seen clearly.
d. The magnification capacity of the microscope
E.the amount of clearance between slide and objective lens.
Chapter 4 Solutions
Biology
Ch. 4.1 - Prob. 1CCCh. 4.1 - Prob. 2CCCh. 4.2 - Prob. 1BCCh. 4.2 - Prob. 1CCCh. 4.2 - Prob. 2BCCh. 4.3 - Prob. 1BCCh. 4.3 - Prob. 1CCCh. 4.3 - Prob. 2BCCh. 4.4 - Prob. 1CCCh. 4.4 - Prob. 1BC
Ch. 4.4 - Prob. 2CCCh. 4.4 - The Nucleus and Endomembrane System Experimental...Ch. 4.4 - Prob. 2EQCh. 4.4 - Prob. 3EQCh. 4.4 - Prob. 3CCCh. 4.5 - Prob. 1CCCh. 4.5 - Core Skill: Connections Look ahead to Figure...Ch. 4.5 - Prob. 2CCCh. 4.6 - Prob. 1CCCh. 4.6 - Prob. 2CCCh. 4 - The cell theory states that a. all living things...Ch. 4 - Prob. 2TYCh. 4 - Prob. 3TYCh. 4 - Prob. 4TYCh. 4 - Each of the following is part of the endomembrane...Ch. 4 - Prob. 6TYCh. 4 - Functions of the smooth endoplasmic reticulum...Ch. 4 - Prob. 8TYCh. 4 - Prob. 9TYCh. 4 - Which of the following observations would not be...Ch. 4 - Prob. 1CQCh. 4 - Explain how motor proteins and cytoskeletal...Ch. 4 - Prob. 3CQCh. 4 - Discuss the roles of the genome and proteome in...Ch. 4 - Prob. 2COQ
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- Both light and electron microscopy are commonly used to visualize cells, cell structures, and the location of specific molecules. Explain why a scientist may choose one or the other microscopy technique for use in research.arrow_forward3D dimensionality is a limitation of the compound microscope. Depth of field, DOF, describes dimensionality form top to bottom and can be observed with colored cross threads. Observe the crossed thread slide on low power (4x), then on medium power (10x), then on high power (40x objective magnification). Which crossed fiber is on top? How do you know?arrow_forwardThe field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?arrow_forward
- Which of the following statements are true in the context of the microscope's field of view? I. It is the observable area within a circular space as defined by the lenses II. FOV is the smallest on the lowest power objective III. FOV is the maximum area visible through the microscope eyepiece IV. When you switch to a higher power, the field of zooms out towards the centerarrow_forwardExplain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?arrow_forwardPlease explain the tablearrow_forward
- In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forwardDyes are used to stain specimens that will be observed using the microscope to a. increase the magnification. b. increase resolution. c. increase contrast. d. make it visible. e. none of the above.arrow_forwarda) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FMarrow_forward
- Previous question: The field of view is the maximum area visible through the lenses of a microscope, and it is represented by a diameter. To determine the diameter of your field of view, you can place a transparent metric ruler under the low power (10X) objective of a microscope. You would focus the microscope on the scale of the ruler and measure the diameter of the field of vision in millimeters. Refer to the image taken of this preparation below. What is the approximate field of view as seen by this 10X objective. Note: This microscope has a 10X eyepiece, giving a total magnification of 100X. The image shows the 1mm is for this question. - Answer for this question was 3mm.arrow_forwardIn microscopy, why are different stains used for different samples? Be specific.arrow_forwardWhat is phase contrast? Give examples of phase contrast in optical microscopy.arrow_forward
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