HUMAN BIOLOGY
HUMAN BIOLOGY
16th Edition
ISBN: 9781260233032
Author: Mader
Publisher: RENT MCG
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Chapter 4, Problem 1TC
Summary Introduction

Introduction:

The patient Kristen was diagnosed with severe burns of the legs. The skin layers consist of the epidermis, dermis and hypodermis. The technique of autografting is used in which the skin from some other parts of the body is transplanted in a region which has been injured and burnt due to injury to the body. If the burn injuries are severe, then skin from some other person is transplanted to the patient with same immunocompatibility so that the chance of rejection decreases. This technique is called as the allograft. The method now a day’s involves the growth and differentiation of the skin cells in a petri plate under aseptic sterile conditions.

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5G JA ATTC 3 3 CTIA A1G5 5 GAAT I I3 3 CTIA AA5 Fig. 5-3: The Eco restriction site (left) would be cleaved at the locations indicated by the arrows. However, a SNP in the position shown in gray (right) would prevent cleavage at this site by EcoRI One of the SNPs in B. rapa is found within the Park14 locus and can be detected by RFLP analysis. The CT polymorphism is found in the intron of the Bra013780 gene found on Chromosome 1. The Park14 allele with the "C" in the SNP has two EcoRI sites and thus is cleaved twice by EcoRI If there is a "T" in that SNP, one of the EcoRI sites is altered, so the Park14 allele with the T in the SNP has only one EcoRI site (Fig. 5-3). Park14 allele with SNP(C) Park14 allele with SNPT) 839 EcoRI 1101 EcoRI 839 EcoRI Fig. 5.4: Schematic restriction maps of the two different Park14 alleles (1316 bp long) of B. rapa. Where on these maps is the CT SNP located? 90 The primers used to amplify the DNA at the Park14 locus (see Fig. 5 and Table 3 of Slankster et…
From your previous experiment, you found that this enhancer activates stripe 2 of eve expression. When you sequence this enhancer you find several binding sites for the gap gene, Giant. To test how Giant interacts with eve, you decide to remove all of the Giant binding sites from the eve enhancer. What results do you expect to see with respect to eve expression?
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HUMAN BIOLOGY

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