Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 4, Problem 1CA
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Your goal is to produce black seeds resistant to mold. So you make the same cross again (between a homozygous black seeded, mold susceptible parent and a homozygous white seeded and mold resistant parent), and, again, advance progeny by SSD to create 100 F10 generation plants.
Based on the information you obtained from your first crossing experiment (Question #4), how many F10 plants would you expect to have black seeds and be resistant to mold?
Assume that a toxin produced by the mold fungus has been isolated. Only mold resistant seeds will germinate in the presence of the toxin. Could you use this toxin screening procedure to have segregation distortion work in your favor in the F2 generation? Explain your answer.
Info from Question 4
a. P Locus (Seed Color):
Hypothesis: The null hypothesis (H₀) is that seed color is controlled by alleles at a single locus.
Observed Data:
Total white seeds: 45 (resistant plants) + 6 (susceptible plants) = 51
Total black seeds: 7 (resistant…
10. Consider the following enzyme and its substrate where the "+" and "-" indicate cations and
anions, respectively. Explain which of the following inhibitors could inhibit this enzyme?
Which type of inhibitor would it be and why? (Video 5-2)
Substrate
Enzyme
Potential inhibitors
Using Punnett Squares Punnett squares are one good
way to predict the outcome of genetic crosses. Punnett
squares use mathematical probability to help predict the
genotype and phenotype combinations in genetic crosses. The
number of possible alleles from each parent determines the
number of rows and columns in the Punnett square.
Independent Assortment
KEY QUESTION How do alleles segregate when more than
one gene is involved?
Mendel wondered if the segregation of one pair of alleles
affects another pair. For example, does the gene that
determines the shape of a seed affect the gene for seed color?
This type of experiment is known as a two-factor, or dihybrid,
cross because it involves two different genes. Single-gene
crosses are monohybrid crosses.
Visual Reading Tool: Two-Factor Cross: F₂
The Punnett square shows the
results of self-crossing the F,
generation of a cross between
round yellow peas and wrinkled
green peas.
1. List the different genotypes in
the F, generation. What is the…
Chapter 4 Solutions
Laboratory Experiments in Microbiology (11th Edition)
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