Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 4, Problem 3Q
What is the purpose of heating the loop before use? After use? _____
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Please help with question. thank you
You are studying the expression of the lac operon. You have isolated mutants as described below. In the presence of glucose, explain/describe what would happen, for each mutant, to the expression of the lac operon when you add lactose AND what would happen when the bacteria has used up all of the lactose (if the mutant is able to use lactose).5. Mutations in the lac operator that strengthen the binding of the lac repressor 200 fold
6. Mutations in the promoter that prevent binding of RNA polymerase
7. Mutations in CRP/CAP protein that prevent binding of cAMP8. Mutations in sigma factor that prevent binding of sigma to core RNA polymerase
Molecular Biology
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A bacteria has a gene whose protein/enzyme product is involved with the synthesis of a lipid necessary for the synthesis of the cell membrane. Expression of this gene requires the binding of a protein (called ACT) to a control sequence (called INC) next to the promoter.
A. Is the expression/regulation of this gene an example of induction or repression?Please explain:B. Is this expression/regulation an example of positive or negative control?C. When the lipid is supplied in the media, the expression of the enzyme is turned off.Describe one likely mechanism for how this “turn off” is accomplished.
Chapter 4 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 4 - What other methods can be used to determine...Ch. 4 - What is the primary use of slants? Of deeps?...Ch. 4 - What is the purpose of heating the loop before...Ch. 4 - Prob. 4QCh. 4 - Why is aseptic technique important?Ch. 4 - Why was the arrangement of Lactococcus in the...Ch. 4 - For a bacterium, what evolutionary advantage is...Ch. 4 - How can you tell that the media provided for this...Ch. 4 - Your microbiology lab maintains reference...
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