Pearson eText for Biochemistry: Concepts and Connections -- Instant Access (Pearson+)
2nd Edition
ISBN: 9780137533114
Author: Dean Appling, Spencer Anthony-Cahill
Publisher: PEARSON+
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Textbook Question
Chapter 4, Problem 11P
DNA from a newly discovered virus was purified, and UV light absorption was followed as the molecule was slowly heated. The absorbance increase at the melting temperature was only 10%. What does this result tell you about the structure of the viral DNA?
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Students have asked these similar questions
If a virus particle contained double-stranded B-DNA of 500,000 base pairs,
A. How many complete helical turns would occur on each strand?
B. How many atoms of phosphorus would be present?
C. What would be the length (in Å) of the DNA before packing into the virus?
Draw a generic (+)RNA virus replication cycle. For viruses, (+) RNA indicates the genomic RNA can be translated directly. How would this differ from the system you drew in #1?
Draw a generic (+) RNA virus replication cycle. Indicate which proteins need to be present for RNA replication and translation. Where would they act?
Indicate what sequences or modifications would need to be present on the virus RNA for RNA replication and translation.
How might a virus differentiate between RNA to be put into progeny virus particles and RNA to be translated into protein?
Assume you isolate a single stranded (+) RNA virus. When you examine the proteins in the virus, you find that it does NOT contain replicase enzymes within its capsid. Which of the following is true?
This virus must have a gene that encodes replicase.
This virus will not be able to enter a host cell.
Its genome cannot be translated (the process of translation) by the host cell ribosomes.
A DNA copy of the viral genome has to be made before viral genes are expressed.
This virus must lack surface antigens.
Chapter 4 Solutions
Pearson eText for Biochemistry: Concepts and Connections -- Instant Access (Pearson+)
Ch. 4 - Prob. 1PCh. 4 - What is the difference between a nucleoside...Ch. 4 - pppApCpCpupApGpApu-OH a. Using the straight-chain...Ch. 4 - Shown is a representation of a molecule being...Ch. 4 - Base analysis of DNA from maize (com) shows it to...Ch. 4 - Using the pKa data in Table 4.1 and the...Ch. 4 - For some DNAs, it is possible to separate the two...Ch. 4 - Refer to Figure 4.15, which presents the...Ch. 4 - Suppose that you centrifuged a transfer RNA...Ch. 4 - Predict the structure of a cruciform that could be...
Ch. 4 - DNA from a newly discovered virus was purified,...Ch. 4 - Would you expect Neurospora crassa DNA to have a...Ch. 4 - A circular double-stranded DNA molecule contains...Ch. 4 - The gel electrophoresis pattern in Figure 4.23 was...Ch. 4 - 15. DNA polymerase requires both a template, to be...Ch. 4 - Prob. 16PCh. 4 - Prob. 17PCh. 4 - a. What two enthalpic factors stabilize DNA in...Ch. 4 - 19.
a. The plasmid pBR322 (4362 base pairs) was...Ch. 4 - Prob. 20PCh. 4 - What DNA sequence feature is required for a...
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- Picture below shows the Hershey and Chase experiment. Identify where viral protein and viral DNA would be located by dragging the labels to the correct targets.arrow_forwardGive detailed Solution with explanation needed (don't copy the answer other sitearrow_forward2) Replicating structures in DNA can be observed in the electron microscope. Regions being replicated appear as bubbles. a) How many replication forks are present? b) Assuming bidirectional replication, how many origins of replication are active in this DNA molecule? c) Assuming that all replication forks move at the same speed, which origin of replication was activated first (left, middle or right)? Why?arrow_forward
- You have begun your career in medicinal biochemistry and have just discovered a bacterial DNA plasmld (transferabl ring of DNA) that appears to destroy the Ebola virus. In order to characterize your new plasmid, the molar mass of the plasmid must be determined. You dissolve 25.00 mg of the purified plasmid in 0.200 mL of water at 2 °C and find the osmotlc pressure of this solution is 1.20 Torr at 20 °C and 1 atm pressure. Answer the following about the Ebola-killing plasmid. 33.) The osmotlc pressure of the system is: (a) 1 atm (b) 0.016 atm (c) 6.5 X 10-5 atm (d) 22.59 atm (e) 0.0016 atmarrow_forwardPrimer needed to initiate DNA replication is A) DNA segment B) RNA segment C) DNA-RNA hybrid D) Nucleotidearrow_forwardWhich of the following is NOT correct concerning the initiation of replication in E. coli? Question 29 options: A) It involves a region of the DNA called oriC. B) DnaA proteins bind to the DNA to begin separation of the strands. C) The strands are initially separated at GC-rich regions of DNA. D) Following initial separation, enzymes continue to separate the parental DNA strands around the rest of the chromosome.arrow_forward
- The dUTPase enzyme is used by the African swine fever virus to repair its own DNA, as the cells the virus infects in swine do not express the dUTPase protein. Which of the following is a reasonable explanation for the role of the dUTPase in the virus lifecycle? Explain your choice in 25 words or less. The dUTPase is necessary to remove dUTP from the viral genome, as uracil should not be present in DNA The dUTPase is necessary to remove dUTP from the viral genome, as uridine cannot correctly base pair with adenine-containing bases The dUTPase is necessary to remove dUTP from the viral genome, as uridine has the wrong sugar component for building DNA More than one of the above answers is correct None of the above answers is correctarrow_forwardYou wish to produce a high-value protein using recombinant DNA technology. Would you try to develop a chemical defined medium or a complex medium? Why? Explain what semiconservative replication means. Give characteristic dimensions for each of these organisms: E. coli Yeast (S. cerevisiae) Liver cell (hepatocyte) Plant cell .What are the differences in cell envelope structure between gram-negative and gram-positive bacteria? These differences become important if you wish to genetically engineer bacteria to excrete proteins into the extracellular fluid.arrow_forwardYou want to digest 1 µg of plasmid DNA in a final volume of 50 μL. Your solution containing plasmid has a concentration of 25 ng/μL. How many μL of your plasmid solution do you need to add to your reaction tube to digest your desired mass of plasmid?arrow_forward
- Figure 1 above shows that remdesivir “mimics” an important component of RNA replication. Which component of RNA replication has a structure similar to that of remdesivir?arrow_forwardYou have recently isolated a virus associated with a human disease. The virus particles are spherical, ~100 nm in diameter, and possess an envelope. Would you expect to find glycoproteins in the viral envelope? yes Would knowing that the particles are spherical mean that they are built with icosahedral symmetry? [Select]arrow_forwardAnswer the following questions. A) Write the protein that catalyzes the formation of phosphodiester bonds. B) Write the protein that breaks the covalent bonds. C) A mutated strain of E. coli normally replicates DNA at 25ºC, but replication immediately stops when the temperature is raised to 37ºC. Which protein is functional at higher temperature? Probably not, please write. D) Write down the enzyme that catalyzes the binding of an amino acid to tRNA in the formation of aminoacyl tRNA. E) Write down the enzyme that is mostly responsible for the synthesis of the chain that begins to form during DNA replication in E. coliarrow_forward
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