CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
12th Edition
ISBN: 9780136486787
Author: Urry
Publisher: SAVVAS L
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Chapter 38, Problem 6TYU
Summary Introduction
Introduction:
Sporophytic incompatibility is a type of self-incompatibility and is commonly defined as the process in which incompatible pollen cannot be germinated on a stigmatic surface. The incompatibility in this can be governed by the genotype of the plant.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 38 Solutions
CAMPBELL BIOLOGY-MASTERING BIO.ACCESS
Ch. 38.1 - Distinguish between pollination and fertilization.Ch. 38.1 - Prob. 2CCCh. 38.1 - MAKE CONNECTIONS Does the life cycle of humans...Ch. 38.2 - What are three ways that flowering plants avoid...Ch. 38.2 - Prob. 2CCCh. 38.2 - Prob. 3CCCh. 38.3 - Compare traditional plant-breeding methods with...Ch. 38.3 - Why does Bt maize have less fumonisin than non-GM...Ch. 38.3 - WHAT IF? In a few species, chloroplast genes are...Ch. 38 - What changes occur to the four types of floral...
Ch. 38 - Prob. 38.2CRCh. 38 - Prob. 38.3CRCh. 38 - A fruit is (A) a mature ovary. (B) a mature ovule....Ch. 38 - Prob. 2TYUCh. 38 - Double fertilization means that (A) flowers must...Ch. 38 - Prob. 4TYUCh. 38 - Prob. 5TYUCh. 38 - Prob. 6TYUCh. 38 - The black dots that cover strawberries are...Ch. 38 - DRAW IT Draw and label the parts of a flower.Ch. 38 - Prob. 9TYUCh. 38 - Prob. 10TYUCh. 38 - SCIENCE, TECHNOLOGY, AND SOCIETY Humans have...Ch. 38 - WRITE ABOUT A THEME: ORGANIZATION In a short essay...Ch. 38 - SYNTHESIZE YOUR KNOWLEDGE This colorized SEM shows...
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