Principles of Biology
Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 37.3, Problem 3TYK
Summary Introduction

Introduction:

Most digestion of food and nutrients absorption occurs in the small intestine. Hydrolytic enzymes break down the organic nutrient macromolecules into monomers. Vitamins and minerals do not need enzymatic digestion and they are easily absorbed in the small intestine. The absorption and digestion are mostly supported by mucosal infolding and specialization along the small intestine length.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
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