Campbell Biology (10th Edition)
Campbell Biology (10th Edition)
10th Edition
ISBN: 9780321775658
Author: Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson
Publisher: PEARSON
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Chapter 37, Problem 8TYU
Summary Introduction

Introduction: Humus is a well decomposed organic material in soil. Humus contain many important nutrients such as nitrogen, phosphorous, magnesium, iron etc.

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You count 54 colonies of bacteria on an agarp late on which you spread 0.2ml of a 10^-4 (or 1/10,000) dilution of soil. Claculate how many colony forming units of bacteria are present. (A) 2700000 (B) 0.0027 (C) 2.6 * 10^-6 (D) 108,000
c) Pasteurization is a technique that uses temperatures of about 72°C to neutralize potential pathogens in foods. a. Justify the effect does this temperature have on the enzymes and cellular metabolism of pathogens. b. Argue why does the heat of pasteurization kill some microorganisms yet fail to affect thermophiles?
A soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.
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