Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 37, Problem 1CT
How would you develop a pure culture of a phage?
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Chapter 37 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 37 - Why did you add Escherichia coli to sewage, which...Ch. 37 - If there were no plaques on your plates, offer an...Ch. 37 - How would you develop a pure culture of a phage?Ch. 37 - How would you isolate a bacteriophage for a...Ch. 37 - Bacteriophages are being explored as a treatment...Ch. 37 - Rotavirus grows in cells at the tips of intestinal...
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- Which of the following experimental techniques was used by Hershey and Chase to establish the genetic material of phage? * None of the above X-ray Isotope labelling Chromatography Ultracentrifugationarrow_forwardIs a phage a bacteria?arrow_forwardYou infect bacteria with two phages during a cis test. This produces a lysate that contains phage particles. Before you can count outcomes, you need to work out the amount of phage to use in subsequent experiments. You start by making two ten- fold serial dilutions of the lysate and then add 1mL of the final diluted lysate to a plate of permissive strain e.coli. You observe 10 plaques. How many phage per mL were present in the original lysate? a. 10 phage/mL o b. 3,300 phage/mL c. 10,000 phage/mL o d. 1,000 phage/mL e. 33 phage/mLarrow_forward
- Which of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forwardYou are attempting to propagate bacteriophage of Bacillus cereus using a liquid batch culture. A growing culture of B. cereus is inoculated with your bacteriophage. The day before you tested the bacteria and the phage batch and they both behaved as expected. You made sure that all containers are labeled appropriately. Immediately after inoculation, you take a sample but are unable to detect any bacteriophage in the media. What is the most likely explanation? O You used the wrong bacteria or the wrong bacteriophage. There is an issue with the phage batch. O There is a problem with your bacterial culture of B. cereus. O It is too soon. The bacteriophages are still replicating and assembling inside the bacterial cells and are therefore not detectable in the growth media.arrow_forwardIn the first step of your phage therapy design process, you isolate the exact Mycobacterium strain from the patient. What is the most important thing must you establish next about this bacterial isolate?arrow_forward
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