Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 37, Problem 2CT
How would you isolate a bacteriophage for a species of Bacillus?
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Chapter 37 Solutions
Laboratory Experiments in Microbiology (11th Edition)
Ch. 37 - Why did you add Escherichia coli to sewage, which...Ch. 37 - If there were no plaques on your plates, offer an...Ch. 37 - How would you develop a pure culture of a phage?Ch. 37 - How would you isolate a bacteriophage for a...Ch. 37 - Bacteriophages are being explored as a treatment...Ch. 37 - Rotavirus grows in cells at the tips of intestinal...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- From what other environments might E. coli bacteriophages be readily isolated?arrow_forward17) What gives Aspergillus sydowii colonies their hairy appearance? a) Hyphae () b) Stipes c) Growing media ) d) Ascocarpoda Jack has designed one primer which is complementary to the DNA of E. coli. He then used that primer for a PCR reaction using DNA isolated from a mixture of bacteria. He got no PC reaction product and hence concluded that there is absolutely no E. coli in the bacteria mixture. His conclusion is .... () True ) Falsearrow_forwardA plasmid that can replicate in E. coli and Pseudomonas is most likely a/ana) broad host range plasmid.b) self-transmissible plasmid.c) high-copy-number plasmid.d) essential plasmid.e) low-copy-number plasmid.arrow_forward
- Can a bacteriophage infect a human?arrow_forwardA plasmid may A) replicate independently of the chromosome. B) be transferred cell-to-cell during conjugation. C) be integrated into the chromosome. D) replicate independently of the chromosome, integrate into the chromosome, or be transferred cell-to-cell during conjugation.arrow_forwardYou want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forward
- If we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)arrow_forwardWhy do we use an Electron Scanning Microscope to see bacteriophages over other microscopes?arrow_forwardWhich of the following statements is not applicable to conjugation? A) OF factors are transferrable plasmids passed from one cell to another during conjugation. B) O During conjugation, rolling circle replication transfers an F factor from an F+ cell to an F- cell. ) O An F factor acquired during conjugation will automatically be integrated into the recipient's chromosome. D) O Conjugation requires physical contact between cells via the formation of a sex pilus. E) O An Hfr strain is capable of transferring a portion of the chromosome during conjugation.arrow_forward
- Use the figure of a plasmid below to answer the following questions. 43) Which enzyme was used to produce the molecule in the figure above? A) RNA polymerase C) a restriction enzyme (endonuclease) B) ligase D) DNA polymerasearrow_forwardCommon vectors used to transfer a piece of DNA into a cloning host are ________. A) artificial chromosomes B) bacteriophages C) All of the choices are correct D) viruses E) plasmidsarrow_forwardAn F' merozygote is formed as a result of the following events: 1) the recipient cell receives genes which used to be a part of an Hfr bacterium chromosome 2) an F' bacterium excuses an F factor from another bacterium chromosome 3) an F factor that was imprecisely excised from Hfr chromosome was transferred to an F- bacterium 4)an F' bacterium conjugate with an F- bacterium 5) an F' bacterium incorporates an F factor into its chromosome 6) an F- bacterium incorporates an F factor into its chromosomearrow_forward
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