1 SEM ACC W/RAVEN CARDED
12th Edition
ISBN: 9781265486297
Author: Raven
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 36.1, Problem 1LO
Predict the direction of water movement based on water potential.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 36 Solutions
1 SEM ACC W/RAVEN CARDED
Ch. 36.1 - Predict the direction of water movement based on...Ch. 36.1 - Prob. 2LOCh. 36.2 - Prob. 1LOCh. 36.2 - Prob. 2LOCh. 36.3 - Prob. 1LOCh. 36.3 - Prob. 2LOCh. 36.4 - Prob. 1LOCh. 36.4 - Prob. 2LOCh. 36.5 - Prob. 1LOCh. 36.6 - Prob. 1LO
Ch. 36.6 - Prob. 2LOCh. 36 - What would w, s, and p of the cell in figure 36.5a...Ch. 36 - Prob. 2DACh. 36 - Data analysis Comparative analyses of fossil and...Ch. 36 - Prob. 1IQCh. 36 - Which of the following is an active transport...Ch. 36 - The water potential of a plant cell is the a. stun...Ch. 36 - Hydrogen bonding between water molecules results...Ch. 36 - Water movement through cell walls is a....Ch. 36 - Prob. 5UCh. 36 - The formation of an air bubble in the xylem is...Ch. 36 - Prob. 7UCh. 36 - Stomata open when guard cells a. take up...Ch. 36 - Prob. 9UCh. 36 - A plant must expend energy to drive a....Ch. 36 - Which of the following statements is inaccurate?...Ch. 36 - If you could override the control mechanisms that...Ch. 36 - Prob. 3ACh. 36 - If you were able to remove the aquaporins from...Ch. 36 - Prob. 5ACh. 36 - If you fertilize your houseplant too often, you...Ch. 36 - How could you detect a plant with a mutation in a...Ch. 36 - Prob. 3SCh. 36 - Prob. 4SCh. 36 - A carrot is a biennial plant. In the first year of...
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