CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 3.6, Problem 4CC
Summary Introduction
To explain: The importance of conjugative plasmid, R plasmid, Col plasmid, and virulence plasmid.
Introduction: A plasmid is a small, extrachromosomal DNA molecule within a cell. It physically separates from chromosomal DNA and it can replicate independently. In bacteria, the plasmids are most commonly found as a small circular, double-stranded DNA molecule. However, sometimes plasmids are present in eukaryotic organisms and archaea.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 3 Solutions
CUSTOM PRESCOTT'S MICROBIOLOGY
Ch. 3.2 - Retrieve, Infer, Apply Why is the term prokaryote...Ch. 3.2 - Retrieve, Infer, Apply What characteristic shapes...Ch. 3.2 - Retrieve, Infer, Apply What advantages might a...Ch. 3.2 - Prob. 4CCCh. 3.3 - Retrieve, Infer, Apply List the functions of...Ch. 3.3 - Prob. 2CCCh. 3.3 - Retrieve, Infer, Apply 3. On what basis are...Ch. 3.3 - Retrieve, Infer, Apply Describe facilitated...Ch. 3.3 - Retrieve, Infer, Apply 5. What are uniport,...Ch. 3.3 - Retrieve, Infer, Apply 6. What are siderophores?...
Ch. 3.4 - MICRO INQUIRY How does the outer membrane of the...Ch. 3.4 - MICRO INQUIRY Are these transporter proteins...Ch. 3.4 - Retrieve, Infer, Apply Describe in detail the...Ch. 3.4 - Retrieve, Infer, Apply When protoplasts and...Ch. 3.4 - Retrieve, Infer, Apply 4. The cell walls of most...Ch. 3.4 - Retrieve, Infer, Apply What two mechanisms allow...Ch. 3.5 - Retrieve, Infer, Apply What is the difference...Ch. 3.5 - Retrieve, Infer, Apply S-layers and some capsules...Ch. 3.6 - Retrieve, Infer, Apply Briefly describe the nature...Ch. 3.6 - Retrieve, Infer, Apply List the most common kinds...Ch. 3.6 - Retrieve, Infer, Apply do plasmids differ from...Ch. 3.6 - Prob. 4CCCh. 3.7 - MICRO INQUIRY How does flagellum growth compare to...Ch. 3.7 - Retrieve, Infer, Apply What are the functions of...Ch. 3.7 - Retrieve, Infer, Apply What terms are used to...Ch. 3.7 - Retrieve, Infer, Apply What is self-assembly? Why...Ch. 3.8 - Would this flagellum be found in a typical...Ch. 3.8 - Retrieve, Infer, Apply Describe the way many...Ch. 3.8 - Prob. 2CCCh. 3.8 - Prob. 3CCCh. 3.8 - Retrieve, Infer, Apply Suggest why chemotaxis is...Ch. 3.9 - Retrieve, Infer, Apply Describe the structure of...Ch. 3.9 - Retrieve, Infer, Apply Briefly describe endospore...Ch. 3.9 - Retrieve, Infer, Apply What features of the...Ch. 3 - Prob. 1RCCh. 3 - Prob. 2RCCh. 3 - Prob. 3RCCh. 3 - Propose a model for the assembly of a flagellum in...Ch. 3 - The peptidoglycan of bacteria has been compared...Ch. 3 - Why might a microbe have more than one uptake...Ch. 3 - Prob. 4ALCh. 3 - What would you expect to observe if you were able...Ch. 3 - Develop a hypothesis to explain why gas vacuoles...Ch. 3 - Prob. 7AL
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