Respiratory system is an organ system that aids in the gaseous exchange in animals and plants. Respiration includes two processes; breathing (external respiration) and cellular respiration (internal respiration). The organs included in the respiratory system are lungs, trachea, nasal cavity, nose, pharynx, larynx, bronchi, bronchioles, and alveoli.

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 35, Problem 7A

Summary Introduction

Introduction:

Respiratory system is an organ system that aids in the gaseous exchange in animals and plants. Respiration includes two processes; breathing (external respiration) and cellular respiration (internal respiration). The organs included in the respiratory system are lungs, trachea, nasal cavity, nose, pharynx, larynx, bronchi, bronchioles, and alveoli.

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Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…

Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 35, Problem 7A

Summary Introduction

Introduction:

Respiratory system is an organ system that aids in the gaseous exchange in animals and plants. Respiration includes two processes; breathing (external respiration) and cellular respiration (internal respiration). The organs included in the respiratory system are lungs, trachea, nasal cavity, nose, pharynx, larynx, bronchi, bronchioles, and alveoli.

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Answer 3

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 35, Problem 7A

Summary Introduction

Introduction:

Respiratory system is an organ system that aids in the gaseous exchange in animals and plants. Respiration includes two processes; breathing (external respiration) and cellular respiration (internal respiration). The organs included in the respiratory system are lungs, trachea, nasal cavity, nose, pharynx, larynx, bronchi, bronchioles, and alveoli.

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Answer 4

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Definition Definition Set of metabolic processes and reactions that produce energy from organic substances such as glucose. Cellular respiration takes place in the organism’s cells, where the chemical energy from nutrients or molecules of oxygen is converted into adenosine triphosphate (ATP). Cellular respiration that occurs in the presence of oxygen is called aerobic respiration; in the absence of oxygen, it is called anaerobic respiration.

Chapter 35, Problem 7A

Summary Introduction