Lab Manual for Biology
12th Edition
ISBN: 9781259298516
Author: Sylvia S. Mader Dr.
Publisher: McGraw-Hill Education
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Chapter 34.1, Problem 1CYP
Summary Introduction
To compare:
The digestive tract of a planarian with that of an earthworm.
Introduction:
Digestion takes place by the absorption of food inside the body of an organism. The planarians include the tapeworms and
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Students have asked these similar questions
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 34 Solutions
Lab Manual for Biology
Ch. 34.1 - Prob. 1CYPCh. 34.1 - 2 Describesome of the limitations of an incomplete...Ch. 34.1 - Prob. 3CYPCh. 34.2 - Prob. 1CYPCh. 34.2 - Describe the likely selective pressuresthat...Ch. 34.2 - Explain how the stomach, small intestine, andlarge...Ch. 34.2 - Prob. 4CYPCh. 34.3 - Prob. 1CYPCh. 34.3 - Prob. 2CYPCh. 34.4 - Prob. 1CYP
Ch. 34.4 - Prob. 2CYPCh. 34.4 - Prob. 3CYPCh. 34 - Prob. 1NS.1QCCh. 34 - Prob. 1NS.2QCCh. 34 - Prob. 2NS.1QCCh. 34 - Prob. 2NS.2QCCh. 34 - Prob. 3NS.1QCCh. 34 - Prob. 3NS.2QCCh. 34 - Prob. 1ACh. 34 - Prob. 2ACh. 34 - Prob. 3ACh. 34 - Prob. 4ACh. 34 - Prob. 5ACh. 34 - Prob. 6ACh. 34 - Prob. 7ACh. 34 - Prob. 8ACh. 34 - Which of these could be absorbed directly without...Ch. 34 - Prob. 10ACh. 34 - Prob. 11ACh. 34 - Prob. 12ACh. 34 - Prob. 13ACh. 34 - Prob. 14ACh. 34 - Prob. 1TSCh. 34 - Prob. 2TSCh. 34 - Prob. 3TSCh. 34 - Prob. 4TS
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