MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
3rd Edition
ISBN: 2810022612789
Author: Cowan
Publisher: MCG
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Chapter 3.4, Problem 1NP
Summary Introduction
Introduction:
Disinfection process is used for the removal of pathogenic bacteria. However, it does not remove all the microbial forms. It cannot remove microbial spores. For example, alcohol can kill pathogenic microbes. It can be applied only to inanimate objects and can reduce the number of bacteria. Alcohol is the best antiseptic that is easily available and inexpensive.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 3 Solutions
MICROBIOLOGY FUND.:CLINICAL APPR-ACCESS
Ch. 3.1 - List the structures all bacteria possess.Ch. 3.1 - Identify three structures some but not all...Ch. 3.1 - Describe three major shapes of bacteria.Ch. 3.1 - Provide at least four terms to describe bacterial...Ch. 3.2 - Describe the structure and function of six...Ch. 3.2 - Prob. 6AYPCh. 3.2 - Q. Device-associated infections are very common...Ch. 3.3 - Differentiate between the two main types of...Ch. 3.3 - Prob. 8AYPCh. 3.3 - Prob. 9AYP
Ch. 3.3 - Prob. 2MMCh. 3.4 - Identify seven structures that may be contained in...Ch. 3.4 - Prob. 11AYPCh. 3.4 - Prob. 1NPCh. 3.5 - Compare and contrast the major features of...Ch. 3.6 - Differentiate between Bergeys Manual of Systematic...Ch. 3.6 - Name four divisions ending in cutes and describe...Ch. 3.6 - Define a species in terms of bacteria.Ch. 3 - Archaea a. are most genetically related to...Ch. 3 - Prob. 2QCh. 3 - Suppose an argument in your city has erupted about...Ch. 3 - Prob. 4QCh. 3 - As a supervisor in the infection control unit, you...Ch. 3 - Prob. 6QCh. 3 - Prob. 7QCh. 3 - Prob. 8QCh. 3 - Bacteria and archaea have a much greater diversity...Ch. 3 - Prob. 10QCh. 3 - Bacteria have been found to change the structures...Ch. 3 - Bacterial and archaeal chromosomes are not...Ch. 3 - Prob. 13QCh. 3 - The results of your patients wound culture just...Ch. 3 - We know that bacteria/archaea and their genetics...Ch. 3 - Find the true statement about biofilms. a. They...Ch. 3 - Suggest more than one reason why bacteria may...Ch. 3 - Construct arguments agreeing with and refuting...Ch. 3 - Which of the following would be used to identify...Ch. 3 - During the cold war between the Soviet Union and...Ch. 3 - During the cold war between the Soviet Union and...Ch. 3 - From chapter 2, figure 2.18. Explain why some...
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