PRINCIPLE OF LIFE (LL)W/ACHIEVE>CUSTOM<
PRINCIPLE OF LIFE (LL)W/ACHIEVE>CUSTOM<
3rd Edition
ISBN: 9781319530709
Author: HILLIS
Publisher: MAC HIGHER
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Chapter 3.4, Problem 1I
Summary Introduction

To explain: At what time the disulfide bonds begin to appear after refolding was allowed.

Introduction: Disulfide bonds are covalent bonds formed in between the cysteine amino acids. They help in the formation of the 3-dimensional structure of the protein. When exposed to denaturation via heating disulfide bonds can break but require subsequent exposure to the chemicals like beta-mercaptoethanol.

Summary Introduction

To explain: The time at which the enzyme activity begins to appear.

Introduction: Enzyme is a protein (mostly, except for ribozymes) made up of amino acids. When a protein is denatured, it loses its structure and hence, its functionality.

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Awnser these Discussion Questions Answer these discussion questions and submit them as part of your lab report. Part A: The Effect of Temperature on Enzyme Activity Graph the volume of oxygen produced against the temperature of the solution. How is the oxygen production in 30 seconds related to the rate of the reaction? At what temperature is the rate of reaction the highest? Lowest? Explain. Why might the enzyme activity decrease at very high temperatures? Why might a high fever be dangerous to humans? What is the optimal temperature for enzymes in the human body? Part B: The Effect of pH on Enzyme Activity Graph the volume of oxygen produced against the pH of the solution. At what pH is the rate of reaction the highest? Lowest? Explain. Why does changing the pH affect the enzyme activity? Research the enzyme catalase. What is its function in the human body? What is the optimal pH for the following enzymes found in the human body? Explain. (catalase, lipase (in your stomach),…
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