CUSTOM PRESCOTT'S MICROBIOLOGY
CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 33.5, Problem 4CC
Summary Introduction

CD4+ T cells and CD8+ T cells are two important T cells present in the human body. T cells are named because they contain cell-surface proteins that possess specific functions in the intercellular communications are known as cluster of differentiation (CDs) molecules or immunogens or antigens. The cluster of differentiation (CDs) molecules are cell-surface proteins and most of them act as receptors. Each molecule of the CD is assigned a specific number designation and that serves to identify cell phenotypes. Till now, over 300 CDs are identified and characterized.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

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CUSTOM PRESCOTT'S MICROBIOLOGY

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