Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Chapter 33.3, Problem 3CC
Summary Introduction
To determine:
What would happen if gastric juice is mixed with crushed food in a test tube.
Introduction:
Gastric juice is secreted by the stomach and it helps in the digestion of the food. Gastric juice contains hydrochloric acid (HCl), mucus, rennin, and pepsin (a protease). It is strongly acidic due to the presence of HCl and has a very low
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Experiment: Action of Gastric Proteases (contains pepsin and renin on proteins)
Procedure:
1. Prepare 3 test tubes and place 2 mL boiled egg albumin solution on each tube.
Tube 1: Add 1 mL Pepsin SolutionTube 2: Add 1 mL of Pepsin + 2 drops conc. HClTube 3: add nothing
2. Keep all the test tubes in a beaker with hot water maintained at 40°C, for one hour or longer.
3. Filter the content of each test tube and perform Biuret’s Test by adding 2 mL 10% NaOH and 2 mL dilute CuSO4 solution to each test tube.
Question: What are the possible observations on each test tube? and What is its inference?
What is the purpose of adding the yogurt to the cooled milk in step 5?
relate the results of assays in terms of the following in animals:
(a) functions of the different sections of the digestive tract,
(b) the presence of various digestive enzymes in each sections or regions, and
(c) the processes that occur in each region.
Chapter 33 Solutions
Campbell Biology in Focus
Ch. 33.1 - An animal requires 20 amino acids to make...Ch. 33.1 - Prob. 2CCCh. 33.1 - WHAT IF? if a zoo animal eating ample food shows...Ch. 33.2 - Prob. 1CCCh. 33.2 - In what sense are nutrients from a recently...Ch. 33.2 - Prob. 3CCCh. 33.3 - How does swallowed food reach the stomach of a...Ch. 33.3 - Explain why a proton pump inhibitor, such as the...Ch. 33.3 - Prob. 3CCCh. 33.4 - Prob. 1CC
Ch. 33.4 - Prob. 2CCCh. 33.4 - Prob. 3CCCh. 33.5 - Prob. 1CCCh. 33.5 - The energy required to maintain each gram of body...Ch. 33.5 - Prob. 3CCCh. 33 - The mammalian trachea and esophagus both connect...Ch. 33 - Which organ is incorrectly paired with its...Ch. 33 - Which of the following is not a major activity of...Ch. 33 - Prob. 4TYUCh. 33 - Prob. 5TYUCh. 33 - If you were to jog 1 km a few hours after lunch,...Ch. 33 - Prob. 7TYUCh. 33 - FOCUS ON EVOLUTION The human esophagus and trachea...Ch. 33 - Prob. 9TYUCh. 33 - Prob. 10TYU
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- Experiment 2: Protein Digestion by Pepsin Label 4 test tubes P1, P2, P3, and P4 (“P” is for Pepsin, a proteolytic enzyme.) To each of the test tubes, add the following, and measure the pH before incubating: Test tube P1: Add 1 mL (1000 ml) albumin + 2 mL (2000 ml) distilled water Record the pH of this tube, here: ______ Test tube P2: Add 1 mL (1000 ml) albumin + 1 mL (1000 ml) HCl + 1 mL (1000 ml) distilled water Record the pH of this tube, here: ______ Test tube P3: Add 1 mL (1000 ml) albumin + 1 mL (1000 ml) pepsin + 1 mL (1000 ml) distilled water Record the pH of this tube, here: ______ Test tube P4: Add 1 mL (1000 ml) albumin + 1 mL HCl (1000 ml) + 1 mL pepsin (1000 ml) Record the pH of this tube, here: ______ 1. Are the results of this experiment consistent with what you know about pepsin? ________________________ 2. List some possible experimental errors that may have influenced your results.arrow_forwarddoes P1000 and P5000 pass validation? Why, why not?arrow_forwardrelate the results of assays in terms of the following in cockroaches: (a) functions of the different sections of the digestive tract, (b) the presence of various digestive enzymes in each sections or regions, and (c) the processes that occur in each region.arrow_forward
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