BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 33, Problem 6P
Interpretation Introduction
Interpretation:
The responsiveness of gene expression for cells treated with estrogen and progesterone should be determined.
Concept introduction:
Recombinant DNA (rDNA) are the DNA strands that are formed by the recombinant method in the laboratories. Recombination is done by bringing together different genes and making a new DNA strand, which otherwise cannot be found in a normal cell’s genome.
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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD.
The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?
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