Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 33, Problem 1FIB
Summary Introduction
Introduction:
In open circulatory system, the blood is not held in blood vessels, rather it circulates through the body cavity, and the cells and tissue are bathed in blood. In the closed circulatory system, blood is present in blood vessels and never gets out in the body cavity.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 33 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 33.1 - explain the major features of all circulatory...Ch. 33.1 - Why doesnt insect hemolymph need hemoglobin?Ch. 33.1 - compare open and closed circulatory systems?Ch. 33.1 - describe the functions of the vertebrate...Ch. 33.2 - Prob. 1CSCCh. 33.2 - describe the three types of vertebrate hearts and...Ch. 33.2 - Prob. 1HYEWCh. 33.2 - Prob. 1TCCh. 33.2 - Prob. 2CYLCh. 33.2 - Prob. 3CYL
Ch. 33.3 - describe each component of blood and explain its...Ch. 33.3 - Prob. 1TCCh. 33.3 - Prob. 2CYLCh. 33.3 - Prob. 2TCCh. 33.3 - explain the sequence of events during blood...Ch. 33.4 - Prob. 1CYLCh. 33.4 - Prob. 1ETCh. 33.4 - Prob. 1TCCh. 33.4 - Prob. 2CYLCh. 33.4 - Prob. 3CYLCh. 33.5 - Prob. 1CSCCh. 33.5 - Prob. 1CSRCh. 33.5 - Prob. 1CYLCh. 33.5 - Prob. 1TCCh. 33.5 - Prob. 2CYLCh. 33.5 - Prob. 3CYLCh. 33 - Prob. 1ACCh. 33 - Prob. 1FIBCh. 33 - Prob. 1MCCh. 33 - List the major structures of all circulatory...Ch. 33 - Prob. 2ACCh. 33 - Prob. 2FIBCh. 33 - Which of the following is True? a. Arteriole...Ch. 33 - Describe and compare the features of open and...Ch. 33 - The hearts pacemaker is called the (complete term)...Ch. 33 - Prob. 3MCCh. 33 - Explain how two- and three-chambered vertebrate...Ch. 33 - Prob. 4FIBCh. 33 - Prob. 4MCCh. 33 - Prob. 4RQCh. 33 - Prob. 5FIBCh. 33 - Which of the following is true of blood pressure?...Ch. 33 - Prob. 5RQCh. 33 - Prob. 6FIBCh. 33 - Prob. 6RQCh. 33 - Lymph is ___________ that has entered lymphatic...Ch. 33 - Prob. 7RQCh. 33 - Prob. 8RQCh. 33 - Describe the cardiac cycle, and relate the...Ch. 33 - Prob. 10RQCh. 33 - Prob. 11RQCh. 33 - Prob. 12RQCh. 33 - In what way do veins and lymphatic vessels...
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