Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 32, Problem 2Q
Summary Introduction
To write:
The advantages and disadvantages of this test over the animal test.
Introduction:
Ames test is an employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. It is a biological assay to access the mutagenic potential of the chemical compounds. It uses several bacterial strains that carry mutations in genes involved.
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Explain how in vivo testing differs from in vitro testing.
Bradford Assay
Fill in the average A595 and A595 of sample minus A595 of blank.
What to do?
Calculating the average absorbance of the standards
Subtracting the absorbance of the blank (the sample with 0 g/ml) from the absorbance of the other samples
Concentration
(micrograms/ml)
A595 -
trial 1
A595 -
trial 2
A595 -
trial 3
average
A595
A595 of sample minus
A595 of blank
0
1.501
1.446
1.447
2
1.624
1.558
1.559
5
1.731
1.749
1.712
10
1.901
1.838
1.892
15
2.161
2.108
2.228
18
2.231
2.277
2.319
What are the shortcomings of randomized clinical trials
Chapter 32 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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