Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 32, Problem 1CT
Summary Introduction
To write:
The Ames test gives a minimum or maximum mutagenic potential.
Introduction:
Ames test is an employed method that uses bacteria to test whether a given chemical can cause mutations in the DNA of the test organism. It is a biological assay to access the mutagenic potential of the chemical compounds. It uses several bacterial strains that carry mutations in genes involved.
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Chapter 32 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
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- Although a large number of mutagenic chemicals are known,none is known that induces mutations in only a single gene(gene-specific mutagenesis). From what you know aboutmutagens, explain why it is unlikely that a gene-specificchemical mutagen will be found. How then is site-specificmutagenesis accomplished?arrow_forwardWhat different between the generated amplification product and the primer sequences in the CDS sequence?arrow_forwardWhat detection method could be used to determine the Mutation at a palindromic site and what results could be achieved from that?arrow_forward
- What is linker scanning mutagenesis?arrow_forwardAfter mutagenesis of wild type Vibrio fisheri, you isolate two different mutant strains (A and B) that, unlike the wild type cells, fail to luminesce when grown to high density in a flask with appropriate medium. Curiously, however, when you inoculate both mutant strains in the same flask, you observe that the mixed (A+B) culture begins to emit light after growing dense. a) What gene/functions are likely affected in each of the two mutants? b) How does this explain their phenotypes?arrow_forwardwhat is thermal stability and explain how the thermal stability of named proteins were improved using site directed mutagenesis.arrow_forward
- "The molecule serving as the genetic material is expected to absorb at the wavelengths shown to be mutagenic." Explain this statement ?arrow_forwardFor the following sequence, what is the Tm? 5'-AGCTACGATCAGGTCA-3'arrow_forwardIntrons in protein-coding genes of some eukaryotes are rarely shorter than 65 nucleotides long. What might be a rationale for this limitation?arrow_forward
- What is transformation efficiency and how is it calculated?arrow_forwardA site-directed mutagenesis experiment was done on the catalytic triad of the serine protease subtilisin where all of the amino acids of the catalytic triad were mutated to alanine. The triple mutant enzyme was characterized kinetically and the mutant displayed a thousand-fold (103) rate enhancement over the uncatalyzed reaction. Explain the source of this rate enhancement. (The native wild-type subtilisin has a rate acceleration of 1010, when compared to the uncatalyzed reaction.)arrow_forwardHow would you modify the Ames test to evaluate physical mutagens?Would it be necessary to add the rat liver extract? Explain why or why not.arrow_forward
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