BIOL 107 LAB PKG W/LAB SG +ACCESS >IC<
12th Edition
ISBN: 9781259827082
Author: Mader
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 31, Problem 1BS.2DQ
Summary Introduction
To determine:
Some factors that could delay the detection of melanoma.
Introduction:
Skin is the main part of the integumentary system. It has two main regions that are epidermis and dermis. Dermis region of the skin is made of “dense connective tissue beneath the epidermis” and contains elastic fibers and collagen. The epidermis of the skin is made up of “stratified squamous epithelium”. Specialized cells that are present in the epidermis contain melanocytes which produce “melanin pigment” responsible for color of skin.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 31 Solutions
BIOL 107 LAB PKG W/LAB SG +ACCESS >IC<
Ch. 31.1 - 1. List five types of epithelium, and identify an...Ch. 31.1 - Prob. 2CYPCh. 31.1 - Prob. 3CYPCh. 31.1 - Recall the three parts of a neuron, and explain...Ch. 31.2 - 1. Explain the difference between an organ and an...Ch. 31.2 - Prob. 2CYPCh. 31.2 - Prob. 3CYPCh. 31.3 - Prob. 1CYPCh. 31.3 - Prob. 2CYPCh. 31.3 - 3. Discuss why a dark-skinned individualliving an...
Ch. 31.3 - Prob. 4CYPCh. 31.4 - Define homeostasis, and discuss why isitimportant...Ch. 31.4 - Explain how the circulatory, respiratory, and...Ch. 31.4 - Prob. 3CYPCh. 31 - Prob. 1NS.1QCCh. 31 - Prob. 1NS.2QCCh. 31 - Prob. 1BS.1DQCh. 31 - Prob. 1BS.2DQCh. 31 - Prob. 1BS.3DQCh. 31 - Prob. 1ACh. 31 - Prob. 2ACh. 31 - Prob. 3ACh. 31 - Prob. 4ACh. 31 - Prob. 5ACh. 31 - Prob. 6ACh. 31 - Prob. 7ACh. 31 - Prob. 8ACh. 31 - Prob. 9ACh. 31 - Prob. 10ACh. 31 - Prob. 11ACh. 31 - Prob. 12ACh. 31 - Prob. 13ACh. 31 - Prob. 14ACh. 31 - Prob. 15ACh. 31 - Prob. 16ACh. 31 - 17. respiratory system
Key:
a. coordinate body...Ch. 31 - Prob. 18ACh. 31 - Prob. 19ACh. 31 - Prob. 20ACh. 31 - Prob. 21ACh. 31 - Prob. 22ACh. 31 - Prob. 23ACh. 31 - Prob. 1TSCh. 31 - Prob. 2TSCh. 31 - Prob. 3TSCh. 31 - Prob. 4TS
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- Which of the following sequencing techniques can identify down to the strain level? O Multilocus sequence typing Genomic fingerprinting Whole genome sequencing OSNP analysis All of the abovearrow_forwardWhat is the "gold standard" that is currently applied to species designations in microbiology? 97% between species: 50% among whole genome 90% between species: 75% among whole genome 99% between species; 97% among whole genome 97% between species: 70% among whole genome Onone of the abovearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have decided to send your sample off for sequencing. You need to determine which type of sequencing to use for the preliminary identification of your species. You decide that, for now, you only need to be able to identify the family and genus levels. Which type of sequencing do you think is the most appropriate? O Genomic Fingerprinting O Whole Genome Sequencing O 16S rDNA Sequencing O DNA-DNA hybridization Nextarrow_forward
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