Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 30, Problem 23P
Interpretation Introduction

(a)

Interpretation:

Nucleolus of 3 different tissue that is brain, liver and muscle are collected and allowed to undergo transcription. The RNA was applied to DNA chips. The cause of intensity of hybridization differing from gene to gene is to be described.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it, due to complementary nature of nucleotides specific polynucleotide strand it will attach to its complementary strands only. The intensity of shading helps in identifying which polynucleotide stands were there in the sample.

Interpretation Introduction

(b)

Interpretation:

The cause of different hybridization pattern of same RNA in different tissue is to be described.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it, due to complementary nature of nucleotides specific polynucleotide strand will attach to its complementary strands only. And the intensity of shading helps in identifying which polynucleotide stands were there in the sample.

Interpretation Introduction

(c)

Interpretation:

The cause of expression of some genes in all the tissues are to be described.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it due to complementary nature of nucleotides specific polynucleotide strand will attach to its complementary strands only. And the intensity of shading helps in identifying which polynucleotide stands were there in the sample.

Interpretation Introduction

(d)

Interpretation:

Cause of addition of inhibition inhibitor in sample is to be proposed.

Concept introduction:

DNA chip technology is also known as the DNA microarray technology. In this process different genes or piece of DNA are collected in a microarray. These genes are attached to a solid surface. When the sample of polynucleotide strand is added upon it due to complementary nature of nucleotides specific polynucleotide strand will attach to its complementary strands only. And the intensity of shading helps in identifying which polynucleotide stands were there in the sample.

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a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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DNA vs RNA (Updated); Author: Amoeba Sisters;https://www.youtube.com/watch?v=JQByjprj_mA;License: Standard youtube license