Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 3, Problem 7E.4RQ
Summary Introduction
To explain: Whether the coffee stain is the biological stain or a simply a compound that is capable of imparting color to the fabric.
Introduction: There are various stains used for the microscopic laboratory procedure. These stains consist of negative ion or positive ion that together forms as a salt. The chromophore of the dyes may be a positive ion or negative ion that defines the nature of the stain. For example, if the stain has positive ion and uncolored counter ion, then that stain is called a basic stain.
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Chapter 3 Solutions
Microbiology: A Laboratory Manual (11th Edition)
Ch. 3 - Why are thick or dense smears less likely to...Ch. 3 - Prob. 6E.2RQCh. 3 - Why should you be careful not to overheat the...Ch. 3 - Why do you think the presence of grease or dirt on...Ch. 3 - Why are basic dyes more effective for bacterial...Ch. 3 - Can simple staining techniques be used to identify...Ch. 3 - Prob. 7E.3RQCh. 3 - Prob. 7E.4RQCh. 3 - Prob. 8E.1RQCh. 3 - Prob. 8E.2RQ
Ch. 3 - Why doesn’t nigrosin penetrate bacterial cells?
Ch. 3 - What are the advantages of differential staining...Ch. 3 - Cite the purpose of each of the following reagents...Ch. 3 - Prob. 9E.3RQCh. 3 - Prob. 9E.4RQCh. 3 - Prob. 9E.5RQCh. 3 - Prob. 10E.1RQCh. 3 - Why do you use acid-alcohol rather than ethyl...Ch. 3 - What is the specific diagnostic value of this...Ch. 3 - Prob. 10E.4RQCh. 3 - A child presents symptoms suggestive of...Ch. 3 - Why is heat necessary in spore staining?
Ch. 3 - Prob. 11E.2RQCh. 3 - Prob. 11E.3RQCh. 3 - Prob. 11E.4RQCh. 3 - Explain the function of copper sulfate in this...
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- With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobinarrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forwardWhich ONE of the following is FALSE regarding haemoglobin? It has two alpha subunits and two beta subunits. The subunits are joined by disulphide bonds. Each subunit covalently binds a haem group. Conformational change in one subunit can be transmitted to another. There are many variant ("mutant") forms of haemoglobin that are not harmful.arrow_forward
- During a routine medical check up of a healthy man it was found that his haematocrit value was highly unusual – value of 60%. What one of the options below is the most likely reason? He will have a diet high in iron. He is likely to be suffering from anaemia. He lives at high altitude. He has recently recovered from an accident where he lost a lot of blood. He has a very large body size.arrow_forwardExplain what age of culture is most likely to produce an endospore?arrow_forwardExplain why hot temperatures greater than 45 degrees celsius would not initiate the sporulation process in endospores?arrow_forward
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