Microbiology: A Laboratory Manual (11th Edition)
11th Edition
ISBN: 9780134098630
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 3, Problem 10E.4RQ
Summary Introduction
To determine: The reason why the application of heat or a surface-active agent is not required during counterstain application in acid-fast staining.
Introduction: Acid-fast staining requires heat when applying a primary stain. This is because heat enhances the penetration of a primary stain through the lipoidal wall and also into the cytoplasm. The counterstain is a stain that contrasts the primary stain, thereby making the stained cells visible easily using a microscope.
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Chapter 3 Solutions
Microbiology: A Laboratory Manual (11th Edition)
Ch. 3 - Why are thick or dense smears less likely to...Ch. 3 - Prob. 6E.2RQCh. 3 - Why should you be careful not to overheat the...Ch. 3 - Why do you think the presence of grease or dirt on...Ch. 3 - Why are basic dyes more effective for bacterial...Ch. 3 - Can simple staining techniques be used to identify...Ch. 3 - Prob. 7E.3RQCh. 3 - Prob. 7E.4RQCh. 3 - Prob. 8E.1RQCh. 3 - Prob. 8E.2RQ
Ch. 3 - Why doesn’t nigrosin penetrate bacterial cells?
Ch. 3 - What are the advantages of differential staining...Ch. 3 - Cite the purpose of each of the following reagents...Ch. 3 - Prob. 9E.3RQCh. 3 - Prob. 9E.4RQCh. 3 - Prob. 9E.5RQCh. 3 - Prob. 10E.1RQCh. 3 - Why do you use acid-alcohol rather than ethyl...Ch. 3 - What is the specific diagnostic value of this...Ch. 3 - Prob. 10E.4RQCh. 3 - A child presents symptoms suggestive of...Ch. 3 - Why is heat necessary in spore staining?
Ch. 3 - Prob. 11E.2RQCh. 3 - Prob. 11E.3RQCh. 3 - Prob. 11E.4RQCh. 3 - Explain the function of copper sulfate in this...
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