The reason why the application of heat or a surface-active agent is not required during counterstain application in acid-fast staining. Introduction: Acid-fast staining requires heat when applying a primary stain. This is because heat enhances the penetration of a primary stain through the lipoidal wall and also into the cytoplasm. The counterstain is a stain that contrasts the primary stain, thereby making the stained cells visible easily using a microscope.

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Chapter 3, Problem 10E.4RQ

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To determine: The reason why the application of heat or a surface-active agent is not required during counterstain application in acid-fast staining.

Introduction: Acid-fast staining requires heat when applying a primary stain. This is because heat enhances the penetration of a primary stain through the lipoidal wall and also into the cytoplasm. The counterstain is a stain that contrasts the primary stain, thereby making the stained cells visible easily using a microscope.

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