Microbiology for Surgical Technologists (MindTap Course List)
2nd Edition
ISBN: 9781111306663
Author: Margaret Rodriguez, Paul Price
Publisher: Cengage Learning
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Chapter 3, Problem 4TBP
Summary Introduction
To describe: The reason why the determination of the antibiotic sensitivity of bacteria takes so long.
Introduction: Antibiotic sensitivity is the vulnerability or susceptibility of bacteria to antibiotics. Antibiotic susceptibility testing is often carried out in clinical practice. This technique is highly useful in the identification of disease-causing bacteria. For example, Staphylococcus saprophyticus.
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19. On the diagram below
a. Label the three pictures as: DNA; polypeptide; or RNA.
b. Label the arrows as: translation or transcription/RNA processing.
c. Add the following details to the diagram.
Promoter region
TATA box
Transcription start site
Transcription terminator
Intron (A,B,C,D)
Exons (1,2,3,4,5)
Splice sites
5' cap
5' UTR (untranslated region)
3' poly A tail
3' UTR (untranslated region)
Translational start (AUG)
Translational stop (UGA, UAG, or UAA)
N and C ends of polypeptide
0000
Match the letter labels in the figure below to the terms. Some letter
labels are not used.
MNNNNNNIN
M
C
B
A
M
D
F
E
H
K
G
8
The diagram below illustrates a quorum sensing pathway from Staphylococcus aureus. Please answer the following questions.
1. Autoinduction is part of the quorum sensing system. Which promoter (P2 or P3) is critical for autoinduction?
2)This staphylococcus aureus grows on human wounds, causing severe infections. You would like to start a clinical trial to treat these wound infections. Please describe:
a) What molecule do you recommend for the trial. Why?
b) Your trial requires that Staphylococcus aureus be isolated from the wound and submitted to genome sequencing before admittance. Why? What are you testing for?
3) If a mutation arises where the Promoter P3 is constitutively active, how would that influence sensitivity to AIP? Please explain your rationale.
4) This pathway is sensitive to bacterial cell density. Describe two separate mutation that would render the pathway active independent of cell density. Briefly explain your rationale.
Mutation 1
Mutation 2
Chapter 3 Solutions
Microbiology for Surgical Technologists (MindTap Course List)
Ch. 3 - Prob. 1TBPCh. 3 - Prob. 2TBPCh. 3 - Prob. 3TBPCh. 3 - Prob. 4TBPCh. 3 - During a bronchoscopy, the surgeon retrieves a...Ch. 3 - During a bronchoscopy, the surgeon retrieves a...Ch. 3 - During a bronchoscopy, the surgeon retrieves a...Ch. 3 - During a bronchoscopy, the surgeon retrieves a...
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