Biochemistry
Biochemistry
9th Edition
ISBN: 9781319114671
Author: Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher: W. H. Freeman
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Chapter 3, Problem 4P
Interpretation Introduction

Interpretation:

The absorbance of a 1 mg/mL solution across a 1-cm path should be determined along with the percentage of transmission of incident-light.

Concept introduction:

The absorbance(A) of a solution is represented as

A=log10(I0/I)

In this equation I0 is the incident-light intensity.

Transmitted-light intensity is denoted by I.

The relation between extinction coefficient, concentration and path length is denoted as l (in cm) as:

A = εlc

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Why the 2nd choice is correct?
a) What are the differences between the Direct & Indirect Immunofluorescence Assays? (0.5 mark) b) What are the advantages of the Indirect Immunofluorescence Assays? (0.5 mark) c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality? (2 marks)
a) What are the differences between the Direct & Indirect Immunofluorescence Assays?  b) What are the advantages of the Indirect Immunofluorescence Assays?  c) A Super-Resolution Imaging Technique was developed in 2018 using imidazole, a His-tag ligand conjugated with a fluorophore to report the presence of a recombinant His-tag protein target, (Sci Rep, 2018, 8:5507). How does this technique improve the image quality?
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