The polyacrylamide gels can be formed in the presence of changing percentages of acrylamide resulting in different pore sizes. The usefulness of different pore sizes should be determined. Concept introduction: SDS-PAGE gel is known as the sodium dodecyl sulfate PAGE. And PAGE is known as the polyacrylamide gel electrophoresis. It is a modification of polyacrylamide gel electrophoresis. It is an analytical technique in biochemistry for the division of charged molecules in mixtures that are based on their molecular masses present in an electric field.

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Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 29P

Interpretation Introduction

Interpretation:

The polyacrylamide gels can be formed in the presence of changing percentages of acrylamide resulting in different pore sizes. The usefulness of different pore sizes should be determined.

Concept introduction:

SDS-PAGE gel is known as the sodium dodecyl sulfate PAGE. And PAGE is known as the polyacrylamide gel electrophoresis. It is a modification of polyacrylamide gel electrophoresis. It is an analytical technique in biochemistry for the division of charged molecules in mixtures that are based on their molecular masses present in an electric field.

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You’ve isolated a protein and determined that the Native molecular weight of the holoenzyme is 160 kD using size exclusion chromatography. Analysis of this protein using SDS-PAGE revealed 2 bands, one at 100 kD and one at 30 kD. The enzyme was found to be 0.829% NAD (by weight). What further can be said regarding the structure of the polypeptide?

Answer 2

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 29P

Interpretation Introduction

Interpretation:

The polyacrylamide gels can be formed in the presence of changing percentages of acrylamide resulting in different pore sizes. The usefulness of different pore sizes should be determined.

Concept introduction:

SDS-PAGE gel is known as the sodium dodecyl sulfate PAGE. And PAGE is known as the polyacrylamide gel electrophoresis. It is a modification of polyacrylamide gel electrophoresis. It is an analytical technique in biochemistry for the division of charged molecules in mixtures that are based on their molecular masses present in an electric field.

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Check out a sample textbook solution

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Answer 3

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 29P

Interpretation Introduction

Interpretation:

The polyacrylamide gels can be formed in the presence of changing percentages of acrylamide resulting in different pore sizes. The usefulness of different pore sizes should be determined.

Concept introduction:

SDS-PAGE gel is known as the sodium dodecyl sulfate PAGE. And PAGE is known as the polyacrylamide gel electrophoresis. It is a modification of polyacrylamide gel electrophoresis. It is an analytical technique in biochemistry for the division of charged molecules in mixtures that are based on their molecular masses present in an electric field.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Definition Definition Chemical process in which one or more monomers combine to produce a very large chain-like molecule called a polymer. The functional groups present on the monomers and their steric effects are responsible for polymerization through a sequence of reactions that vary in complexity. There exists a stable covalent chemical bond between monomers that sets apart polymerization from other processes.

Chapter 3, Problem 29P

Interpretation Introduction