WHAT IS LIFE? GDE.TO BIO.W/PHYS.-ACCESS
WHAT IS LIFE? GDE.TO BIO.W/PHYS.-ACCESS
5th Edition
ISBN: 9781319360719
Author: PHELAN
Publisher: MAC HIGHER
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Chapter 3, Problem 2MC
Summary Introduction

Introduction:

Disaccharides are carbohydrates that can be broken into two basic sugar units called monosaccharides.

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If PCR was performed on the fragment of DNA shown below using "5'-TAGG-3" and "3'-TCTA-5'" as the primers, how many base pairs long would the PCR product be? To help with this, remember the antiparallel structure of DNA and that primers are complementary and antiparallel to the target sequence that they bind to. Hint: Check out the 5' and 3' labels....they are important! 3’- T  A  T  C  C  G  A  C  A  A  T  C  G  A  T  C  G  A  T  T  G  C  C  T  T  C  T  A  A -5’ 5’- A  T  A  G  G  C  T  G  T  T  A  G  C  T  A  G  C  T  A  A  C  G  G  A  A  G A  T  T – 3’
When setting up a PCR reaction to act as a negative control for the surface protein A gene... Which primers will you add to the reaction mix? mecA primers, spa primers, mecA primers and spa primers, no primers What will you add in place of template? sterile water, MRSA DNA, Patient DNA, S. aureus DNA
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