BIOCHEMISTRY
BIOCHEMISTRY
9th Edition
ISBN: 2818440090622
Author: BERG
Publisher: MAC HIGHER
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Chapter 3, Problem 28P
Interpretation Introduction

Interpretation:

In the given experiment, the observation needs to be explained, if the given protein does not contain any disulfide bond and if it contains a single disulfide bond. An experiment to identify the cysteine residue containing the disulfide bond needs to be explained.

Concept introduction:

2-D electrophoresis is a technique that is used to analyze proteins. Here mixture of proteins is separated based on two properties in two dimensions of the same gel. Normally, in the first dimension proteins are separated according to the isoelectric point and then the proteins are separated in a perpendicular dimension based on their mass.

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Biomolecules - Protein - Amino acids; Author: Tutorials Point (India) Ltd.;https://www.youtube.com/watch?v=ySNVPDHJ0ek;License: Standard YouTube License, CC-BY