Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
3rd Edition
ISBN: 9780134895727
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky
Publisher: PEARSON
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Chapter 29.4, Problem 3CC
Summary Introduction
To explain:
The yellowing of the older leaves of soybean plant followed by a long wet weather.
Introduction:
The nitrogen fixing bacteria, such as Rhizobium, Klebsiella, and Frankia forms the symbiotic relationship with plant species (legumes). They benefit the plants by reducing the nitrogen gas into ammonia. The leguminous species are benefited by the soil bacteria by making nitrogen in the available form for the absorption.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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Chapter 29 Solutions
Campbell Biology In Focus, Loose-leaf Edition (3rd Edition)
Ch. 29.1 - Why is long-distance transport important for...Ch. 29.1 - Prob. 2CCCh. 29.1 - WHAT IF? Some plants can detect increased levels...Ch. 29.2 - If a plant cell immersed in distilled water has a...Ch. 29.2 - How would a reduction in the number of aquaporin...Ch. 29.2 - WHAT IF? What would happen if you put plant...Ch. 29.3 - Prob. 1CCCh. 29.3 - WHAT IF? If an element increases the growth rate...Ch. 29.3 - Prob. 3CCCh. 29.4 - Prob. 1CC
Ch. 29.4 - Prob. 2CCCh. 29.4 - Prob. 3CCCh. 29.5 - A scientist adds a water-soluble inhibitor of...Ch. 29.5 - WHAT IF? Suppose an Arabidopsis mutant lacking...Ch. 29.5 - Prob. 3CCCh. 29.6 - Prob. 1CCCh. 29.6 - WHAT IF? If you buy cut flowers, why might the...Ch. 29.6 - Prob. 3CCCh. 29.7 - Prob. 1CCCh. 29.7 - Prob. 2CCCh. 29.7 - Prob. 3CCCh. 29 - Which structure or compartment is part of the...Ch. 29 - Prob. 2TYUCh. 29 - Prob. 3TYUCh. 29 - What would enhance water uptake by a plant cell?...Ch. 29 - Prob. 5TYUCh. 29 - Compared with a cell with few aquaporin proteins...Ch. 29 - Two groups of tomatoes were grown in the...Ch. 29 - Prob. 8TYUCh. 29 - Prob. 9TYUCh. 29 - SCIENTIFIC INQUIRY A Minnesota gardener notes that...Ch. 29 - Prob. 11TYUCh. 29 - FOCUS ON INTERACTIONS The soil in which plants...Ch. 29 - Prob. 13TYU
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- Which of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forwardWhat is the most appropriate purpose of building a phylogenetic tree? They look awesome You can use a tree to compare morphological characteristics of organisms It can be used to establish and analyze evolutionary relationships between species All of the abovearrow_forward
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