PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 29.2, Problem 2CC
Summary Introduction
Certain molecular techniques are helpful to isolate or identify the desired microbes from microbial diversity. The isolation of microbes is important to obtain a single microbe in the population. The isolation is important to separate the desired microbes from the rest of the mixed population.
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A unique aquatic plant was discovered from a lagoon in El Nido,
Palawan. To determine the protein content of the plant, an
adequate amount of the plant extract was acquired, and Bradford
assay was performed. Determine the total protein concentration,
in µg/mL, of the acquired plant extract. A bovine serum albumin
(BSA) stock solution with a concentration of 250 µg/mL was used
and mixtures with the following compositions and absorbance
readings were prepared:
Volume of BSA
(mL)
Volume of water
(mL)
Absorbance
Tube #
at 595 nm
1
0.00
2.00
0.000
2
0.20
1.80
0.112
3
0.40
1.60
0.225
4
0.60
1.40
0.318
5
0.80
1.20
0.432
6
1.00
1.00
0.551
The absorbance reading of the plant extract is 0.275.
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Chapter 29 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 29.1 - What happens to the flow stream in fluorescence...Ch. 29.1 - Prob. 1CCCh. 29.1 - Prob. 2CCCh. 29.1 - Describe the rationale upon which the extinction...Ch. 29.1 - Prob. 4CCCh. 29.2 - Prob. 1MICh. 29.2 - Prob. 1CCCh. 29.2 - Prob. 2CCCh. 29.2 - Prob. 3CCCh. 29.2 - Prob. 4CC
Ch. 29.3 - How do radioactive and stable isotopes differ?...Ch. 29.3 - Prob. 2CCCh. 29.3 - Prob. 3CCCh. 29.3 - Prob. 4CCCh. 29.3 - What is the difference between MAR and MAR-FISH?...Ch. 29 - Prob. 1RCCh. 29 - Prob. 2RCCh. 29 - Prob. 3RCCh. 29 - Prob. 4RCCh. 29 - Prob. 5RCCh. 29 - Prob. 6RCCh. 29 - Prob. 7RCCh. 29 - Prob. 8RCCh. 29 - Prob. 9RCCh. 29 - Prob. 10RCCh. 29 - Prob. 11RCCh. 29 - How might you attempt to grow in the laboratory a...Ch. 29 - Both acetate and CO2 can be used by methanogenic...Ch. 29 - Prob. 3AL
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