PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 29.1, Problem 1MI
What happens to the flow stream in fluorescence activated cell sorting (FACS)?
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Chapter 29 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 29.1 - What happens to the flow stream in fluorescence...Ch. 29.1 - Prob. 1CCCh. 29.1 - Prob. 2CCCh. 29.1 - Describe the rationale upon which the extinction...Ch. 29.1 - Prob. 4CCCh. 29.2 - Prob. 1MICh. 29.2 - Prob. 1CCCh. 29.2 - Prob. 2CCCh. 29.2 - Prob. 3CCCh. 29.2 - Prob. 4CC
Ch. 29.3 - How do radioactive and stable isotopes differ?...Ch. 29.3 - Prob. 2CCCh. 29.3 - Prob. 3CCCh. 29.3 - Prob. 4CCCh. 29.3 - What is the difference between MAR and MAR-FISH?...Ch. 29 - Prob. 1RCCh. 29 - Prob. 2RCCh. 29 - Prob. 3RCCh. 29 - Prob. 4RCCh. 29 - Prob. 5RCCh. 29 - Prob. 6RCCh. 29 - Prob. 7RCCh. 29 - Prob. 8RCCh. 29 - Prob. 9RCCh. 29 - Prob. 10RCCh. 29 - Prob. 11RCCh. 29 - How might you attempt to grow in the laboratory a...Ch. 29 - Both acetate and CO2 can be used by methanogenic...Ch. 29 - Prob. 3AL
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- 5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion rate of membrane proteins. You attach a fluorescent marker to your protein of interest, bleach a small region of the cell membrane with an intense laser light, and determine the time it takes for the bleached spot to recover fluorescent signal (see figure below). (a) (b) Bleach Laser bleaching of fluorescent marker Fluorescence intensity recovery Recovery Time How would the recovery time of your protein change if the membrane contained a higher concentration of unsaturated fatty acids? Why? How would the recovery time of your protein change if you conducted the experiment at a lower temperature? Why? How would the recovery time of your protein change if it were anchored to the membrane skeleton? Why?arrow_forwardFluorescence-activated cell sorting (FACS) is a powerful technique for separating cells according to their content of particular molecules. For example, a fluorescence-labeled antibody specific for a cell-surface protein can be used to detect cells containing such a molecule. Suppose that you want to isolate cells that possess a receptor enabling them to detect bacterial degradation products. However, you do not yet have an antibody directed against this receptor. Which fluorescence-labeled molecule would you prepare to identify such cells?arrow_forwardWhat are prophase1?arrow_forward
- Why is Neurospora used as genetic material?arrow_forwardThe nucleus is the largest of the eukaryotic organelles and contains the genome of the organism. a) describe the proteins and process of nuclear import and export. b) describe, in general terms, an experiment that could be conducted to show that there are specific signals for nuclear import or export.arrow_forwardHow are integrins activated and how do they control cell proliferation? Regarding the cell-matrix adhesions they form, how do these adhesions respond to mechanical force?arrow_forward
- You treat some cancer cells with a new therapeutic that you hope will kill them. You run an MTT assay on the results and notice that the sample from the control cells (not treated with drug) turns purple upon addition of the MTT and the sample from the cells treated your drug are clear after adding MTT. As you walk down the hall to run your sample on the spectrophotometer, how are you feeling? (a) frustrated, (b) unsure, (c) excited. I understand that if the cell are alive, they will have functioning mitochondria, the mitochondria will be producing NADH and NADPH which will allow some enzymes in the cell to convert empty T which is colorless, into a colored form as and die. The colorelss means less dye with present, which means the less empty T was converted, meaning that we had less mitochondrial activity, which means we had less live cells. I think that the answer should be (a) frustrated. I am not sure if I am right. Glad if the expert advise.arrow_forwardWhat are ROS and examples of ROS? How do they affect the cells resulting in aging?arrow_forwardWhat is the expansion of acronym MICA?arrow_forward
- What are the basic properties of e-cadherins? Namely, what is their basic structure, what affects their function (e.g. Ca2+), their ligand activity, and their role in cell sorting?arrow_forward(c) What are mitotic disrupters? Explain with an example. mitotic disruptersarrow_forwardFigure 5: MCF-7 cells were incubated for 24 h with the drug dilution buffer (control) or various concentrations of indibulin. They were then stained with an antibody against α-tubulin (red channel), and Hoechst 33258 (blue channel). Scale bar = 10 μm. Question : Comment Figure 5arrow_forward
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