ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
4th Edition
ISBN: 9781265061173
Author: McKinley
Publisher: MCG CUSTOM
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Chapter 29, Problem 9DYKB
_____ 9. Skin color is a trait that is determined by
- a. strict dominant-recessive inheritance.
- b. incomplete dominant inheritance.
- c. codominant inheritance.
- d. polygenic inheritance.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 29 Solutions
ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
Ch. 29.1 - What distinguishes the pre-embryonic period from...Ch. 29.2 - Prob. 2WDYLCh. 29.2 - Prob. 3WDYLCh. 29.2 - How many cells are present initially in the...Ch. 29.2 - Prob. 5WDYLCh. 29.2 - Prob. 6WDYLCh. 29.2 - Prob. 7WDYLCh. 29.2 - What are the two cell layers of the bilaminar...Ch. 29.2 - Which cell layers give rise to each of the three...Ch. 29.2 - Prob. 10WDYL
Ch. 29.3 - Prob. 11WDYLCh. 29.3 - Prob. 12WDYLCh. 29.3 - Prob. 13WDYLCh. 29.3 - Why is it important for a pregnant woman to...Ch. 29.4 - Prob. 15WDYLCh. 29.5 - Prob. 16WDYLCh. 29.5 - How do estrogen and progesterone act to sustain...Ch. 29.5 - What are the actions of CRH, HPL, oxytocin, and...Ch. 29.5 - Prob. 19WDYLCh. 29.5 - Prob. 20WDYLCh. 29.5 - Prob. 21WDYLCh. 29.5 - Prob. 22WDYLCh. 29.5 - Prob. 23WDYLCh. 29.5 - Prob. 24WDYLCh. 29.5 - Prob. 25WDYLCh. 29.6 - How do progesterone, estrogen, and oxytocin...Ch. 29.6 - Prob. 27WDYLCh. 29.6 - Prob. 28WDYLCh. 29.6 - Prob. 29WDYLCh. 29.6 - Prob. 30WDYLCh. 29.6 - Prob. 31WDYLCh. 29.7 - Prob. 32WDYLCh. 29.7 - Prob. 33WDYLCh. 29.8 - Prob. 34WDYLCh. 29.8 - Prob. 35WDYLCh. 29.8 - How does the positive feedback mechanism in...Ch. 29.8 - Prob. 37WDYLCh. 29.9 - Prob. 38WDYLCh. 29.9 - How does codominant inheritance differ from...Ch. 29.9 - Prob. 40WDYLCh. 29.9 - Prob. 41WDYLCh. 29 - _____ 1. The outer layer of the blastocyst that...Ch. 29 - _____ 2. At about day 3 after fertilization, the...Ch. 29 - During gastrulation, cells from the _____ layer of...Ch. 29 - ______ 4. The cells of the embryoblast...Ch. 29 - _____ 5. Which of the following is not an...Ch. 29 - _____ 6. All of the following cardiovascular...Ch. 29 - _____ 7. After a woman gives birth, what happens...Ch. 29 - _____ 8. Freckles are considered to be a dominant...Ch. 29 - _____ 9. Skin color is a trait that is determined...Ch. 29 - A woman is a carrier for the color-blindness gene,...Ch. 29 - Briefly describe the process of fertilization,...Ch. 29 - List the five regions of the mesoderm, and...Ch. 29 - Explain why teratogens are especially harmful to...Ch. 29 - Describe the differences between the embryonic...Ch. 29 - Prob. 15DYKBCh. 29 - Prob. 16DYKBCh. 29 - Prob. 17DYKBCh. 29 - Describe the various ways by which the mothers...Ch. 29 - Compare and contrast strict dominant-recessive...Ch. 29 - Explain the difference between X-linked recessive...Ch. 29 - Prob. 1CALCh. 29 - Ashley is a 29-year-old pregnant woman who is in...Ch. 29 - Ashley is a 29-year-old pregnant woman who is in...Ch. 29 - Prob. 4CALCh. 29 - Ashley is a 29-year-old pregnant woman who is in...Ch. 29 - Prob. 1CSLCh. 29 - Prob. 2CSLCh. 29 - Prob. 3CSL
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