Principles of Anatomy and Physiology
Principles of Anatomy and Physiology
14th Edition
ISBN: 9781118345009
Author: Gerard J. Tortora
Publisher: WILEY
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Chapter 29, Problem 20CP
Summary Introduction

To review:

The biological terminology, “limb buds.”

Introduction:

Basic limb structure gets established in the second half of the embryo development, but the initial structure of limb is initiated in the fourth week and is known as limb bud.

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You aim to test the hypothesis that the Tbx4 and Tbx5 genes inhibit each other's expression during limb development. With access to chicken embryos and viruses capable of overexpressing Tbx4 and Tbx5, describe an experiment to investigate whether these genes suppress each other's expression in the limb buds. What results would you expect if they do repress each other? What results would you expect if they do not repress each other?
You decide to delete Fgf4 and Fgf8 specifically in the limb bud. Explain why you would not knock out these genes in the entire embryo instead.
You implant an FGF10-coated bead into the anterior flank of a chicken embryo, directly below the level of the wing bud. What is the phenotype of the resulting ectopic limb?  Briefly describe the expected expression domains of 1) Shh, 2) Tbx4, and 3) Tbx5 in the resulting ectopic limb bud.

Chapter 29 Solutions

Principles of Anatomy and Physiology

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General Embryology Review in 20 minutes; Author: Medical Animations;https://www.youtube.com/watch?v=4YKvVeVMmEE;License: Standard youtube license