The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated. Concept introduction: DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

Suppose the data below are obtained for an enzyme catalyzed reaction with and without the inhibitor I. (s)( mM) 0.2 0.4 0.8 1.0 2.0 4.0 V without i (mM/min) 5.0 7.5 10.0 10.7 12.5 13.6 V with I (mM/min) 3.0 5.0 7.5 8.3 10.7 12.5 Make a Lineweaver Burke plot for this data using graph paper or a spreadsheet Calculate KM and Vmax without inhibitor. What type of inhibition is observed? show graph and work 2. Give the Lineweaver Burk equation and define all the parameters. 3. When substrate concentration is much greater than Km, the rate of catalysis is almost equal to a. kcat b. none of these c. all of these d. Kd e. Vmax

Please explain the process of how an axon degenerates in the central nervous system following injury and how it affects the neuron/cell body, as well as presynaptic and postsynaptic neurons. Explain processes such as chromatolysis and how neurotrophin signaling works.

Please help determine the Relative Response Ratio of my GC-MS laboratory: Laboratory: Alcohol Content in Hand Sanditizers Internal Standard: Butanol Standards of Alcohols: Methanol, Ethanol, Isopropyl, n-Propanol, Butanol Recorded Retention Times: 0.645, 0.692, 0.737, 0.853, 0.977 Formula: [ (Aanalyte / Canalyte) / (AIS / CIS) ]

Answer 2

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 6

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 7

Chapter 29, Problem 1P

Interpretation Introduction

Interpretation:

The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.

Concept introduction:

DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.

Answer 8

Expert Solution & Answer

Answer to Problem 1P

The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Explanation of Solution

It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.

The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.

The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to 5' phosphate and the leaving group present in this enzyme is adenosine monophosphate(AMP).

The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which 5' phosphate is attached to phenolic −OH group and the leaving group present in this enzyme is the tyrosine residue.

Answer 12

Conclusion

The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.

Answer 13

Ch. 29 - Prob. 1P Ch. 29 - Prob. 2P Ch. 29 - Prob. 3P Ch. 29 - Prob. 4P Ch. 29 - Prob. 5P Ch. 29 - Prob. 6P Ch. 29 - Prob. 7P Ch. 29 - Prob. 8P Ch. 29 - Prob. 9P Ch. 29 - Prob. 10P

Concept explainers

Videos

Answer to Problem 1P

Explanation of Solution

Want to see more full solutions like this?

Chapter 29 Solutions