To discuss:
Other applications of Agrobacterium mediated plant transformation.
Introduction:
Plant transformation is a scientific approach whereby DNA from any organism is inserted into the genome of a plant species of interest. The inserted DNA is known as a “transgene”, and the plant is said to be “transgenic”. Transgenic plants are vital for both research and agriculture for the development of new strains.
Agrobacterium tumefaciens is a Gram-negative organism that infects plants. It possesses a Ti plasmid that is a circular piece of DNA capable of autonomous replication. The introduction of the plasmid causes the development of tumors known as crown gall. The genetic material that is introduced is known as T DNA (transferred DNA). It is possible to use the system of the Ti plasmid to remove the genes for crown gall formation with genes of our interest to be expressed in the plants.
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