ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
4th Edition
ISBN: 9781265061173
Author: McKinley
Publisher: MCG CUSTOM
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Chapter 28.3, Problem 23WDYL
Summary Introduction
To explain: The effects of prolactin and oxytocin on female mammary glands.
Concept introduction:
The mammary glands are located in the anterior thoracic wall. Mammary glands are comprised of the tubuloalveolar exocrine gland. The mammary glands are secretory glands and secrete milk which consists of lactose, fats, and proteins.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 28 Solutions
ANATOMY & PHYSIOLOGY 4/E PAC 1 SEMESTER
Ch. 28.1 - What general components make up the reproductive...Ch. 28.1 - What hormones begin to be secreted at puberty, and...Ch. 28.1 - What are the components of the urogenital...Ch. 28.2 - How do sex chromosomes differ from autosomes?Ch. 28.2 - Prob. 5WDYLCh. 28.2 - Prob. 6WDYLCh. 28.2 - Prob. 7WDYLCh. 28.2 - Prob. 8WDYLCh. 28.2 - Prob. 9WDYLCh. 28.2 - Prob. 10WDYL
Ch. 28.3 - Prob. 11WDYLCh. 28.3 - Prob. 12WDYLCh. 28.3 - Prob. 13WDYLCh. 28.3 - Prob. 14WDYLCh. 28.3 - What are the three phases of the ovarian cycle,...Ch. 28.3 - What are the four segments of the uterine tubes,...Ch. 28.3 - Prob. 17WDYLCh. 28.3 - Prob. 18WDYLCh. 28.3 - What are the three phases of the uterine cycle,...Ch. 28.3 - Compare and contrast the ovarian and uterine...Ch. 28.3 - What are the individual components of the female...Ch. 28.3 - Prob. 22WDYLCh. 28.3 - Prob. 23WDYLCh. 28.3 - Prob. 24WDYLCh. 28.4 - How does the scrotum help regulate the temperature...Ch. 28.4 - What are the major cell types in the seminiferous...Ch. 28.4 - What hormones are produced by the interstitial...Ch. 28.4 - How does a spermatogonium divide to produce...Ch. 28.4 - Prob. 29WDYLCh. 28.4 - Prob. 30WDYLCh. 28.4 - What are the specific functions of the accessory...Ch. 28.4 - How is seminal fluid different from semen?Ch. 28.4 - What are the similarities and differences between...Ch. 28.4 - Prob. 34WDYLCh. 28.4 - Prob. 35WDYLCh. 28.5 - Prob. 36WDYLCh. 28.5 - Prob. 37WDYLCh. 28.5 - Prob. 38WDYLCh. 28.5 - Prob. 39WDYLCh. 28.5 - Prob. 40WDYLCh. 28.5 - What factors affect the age that menarche first...Ch. 28.5 - Prob. 42WDYLCh. 28 - _____ 1. The female homologue to the glans of the...Ch. 28 - _____ 2. Ovulation occurs due to a dramatic peak...Ch. 28 - _____ 3. Which statement is accurate about the...Ch. 28 - _____ 4. Which structure contains a primary...Ch. 28 - _____ 5. In the male, what cells produce...Ch. 28 - Prob. 6DYKBCh. 28 - _____ 7. Spermatogonia divide by mitosis to form a...Ch. 28 - _____ 8. Sperm are stored in the _____, where they...Ch. 28 - _____ 9. Which statement is accurate about the...Ch. 28 - _____ 10. The paramesonephric ducts in the embryo...Ch. 28 - What are some anatomic similarities between the...Ch. 28 - What hormones are associated with the female...Ch. 28 - Prob. 13DYKBCh. 28 - List the uterine wall layers, and describe the...Ch. 28 - Compare and contrast the ovarian cycle phases and...Ch. 28 - Describe the relationship among the hypothalamus,...Ch. 28 - What is the function of sustentacular cells in the...Ch. 28 - Describe the process of spermatogenesis, including...Ch. 28 - How do erection and ejaculation occur in the male?Ch. 28 - What structures are formed from the...Ch. 28 - Prob. 1CALCh. 28 - Prob. 2CALCh. 28 - The physician reviews Luisas and Victors blood...Ch. 28 - Prob. 4CALCh. 28 - Prob. 5CALCh. 28 - Prob. 1CSLCh. 28 - Caitlyn had unprotected sex with her fianc...Ch. 28 - Prob. 3CSL
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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