CUSTOM PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 9781266032844
Author: WILLEY
Publisher: MCG
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Chapter 28.1, Problem 2.3CC
Summary Introduction
The growth or development of all living organisms depends upon the obtainability of mineral nutrients. Nitrogen is a mineral and also a crucial constituent of the bodies of all the living organisms as the nitrogen atoms are present in DNA and all proteins. Nitrogen occurs in many forms. In addition, it is cycled continuously by a wide range of bacteria. The cycling of nitrogen in various forms is considered as a complex process and it involves five steps. They are nitrogen fixation, nitrification, assimilation, ammonification, and denitrification.
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A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
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Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
A sample of blood was taken from the above individual and prepared for haemoglobin analysis. However, when water was added the cells did not lyse and looked normal in size and shape. The technician suspected that they had may have made an error in the protocol – what is the most likely explanation?
The cell membranes are more resistant than normal.
An isotonic solution had been added instead of water.
A solution of 0.1 M NaCl had been added instead of water.
Not enough water had been added to the red blood cell pellet.
The man had sickle-cell anaemia.
With reference to their absorption spectra of the oxy haemoglobin intact line) and deoxyhemoglobin (broken line) shown in Figure 2 below, how would you best explain the reason why there are differences in the major peaks of the spectra? Figure 2. SPECTRA OF OXYGENATED AND DEOXYGENATED HAEMOGLOBIN OBTAINED WITH THE RECORDING SPECTROPHOTOMETER 1.4 Abs < 0.8 06 0.4 400 420 440 460 480 500 520 540 560 580 600 nm 1. The difference in the spectra is due to a pH change in the deoxy-haemoglobin due to uptake of CO2- 2. There is more oxygen-carrying plasma in the oxy-haemoglobin sample. 3. The change in Mr due to oxygen binding causes the oxy haemoglobin to have a higher absorbance peak. 4. Oxy-haemoglobin is contaminated by carbaminohemoglobin, and therefore has a higher absorbance peak 5. Oxy-haemoglobin absorbs more light of blue wavelengths and less of red wavelengths than deoxy-haemoglobin
Chapter 28 Solutions
CUSTOM PRESCOTT'S MICROBIOLOGY
Ch. 28.1 - Prob. 1MICh. 28.1 - What microbes are capable of methanogenesis and...Ch. 28.1 - Prob. 1.1CCCh. 28.1 - Prob. 1.2CCCh. 28.1 - Prob. 1.3CCCh. 28.1 - Prob. 1.4CCCh. 28.1 - Prob. 1.5CCCh. 28.1 - What are the products of denitrification,...Ch. 28.1 - Prob. 2.1CCCh. 28.1 - Prob. 2.2CC
Ch. 28.1 - Prob. 2.3CCCh. 28.1 - Prob. 2.4CCCh. 28.1 - Prob. 4MICh. 28.1 - Prob. 5MICh. 28.1 - Compare the products of dissimilatory sulfate...Ch. 28.1 - Prob. 3.2CCCh. 28.1 - Prob. 3.3CCCh. 28.1 - Prob. 3.4CCCh. 28.1 - Suggest how a chemolithoautotroph might link the...Ch. 28.2 - What organisms benefit from nitrification?Ch. 28.2 - List three greenhouse gases. Discuss their...Ch. 28.2 - Prob. 2CCCh. 28.2 - How do changes in the nitrogen cycle caused by...Ch. 28.2 - Prob. 4CCCh. 28 - Prob. 1RCCh. 28 - Prob. 2RCCh. 28 - Prob. 3RCCh. 28 - Prob. 5RCCh. 28 - Prob. 6RCCh. 28 - Prob. 7RCCh. 28 - Prob. 8RCCh. 28 - Prob. 1ALCh. 28 - A bacterium isolated from sewage sludge was...Ch. 28 - Prob. 3ALCh. 28 - Prob. 4AL
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