Study Guide With Student Solutions Manual And Problems Book For Garrett/grisham's Biochemistry, 6th
6th Edition
ISBN: 9781305882409
Author: GARRETT, Reginald H.; Grisham, Charles M.
Publisher: Brooks Cole
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Chapter 28, Problem 8P
Human Genome Replication Rate Assume
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Examine the metabolic pathway. The enzymes that catalyze each step are identified as "e" with a numeric subscript.
e₁
e3
e4
A
B
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1°
B'
02
e5
e6
e7
E
F
Which enzymes catalyze irreversible reactions?
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e4
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es
26
5
e7
Which of the enzymes is likely to be the allosteric
enzyme that controls the synthesis of G?
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e4
es
26
5
e7
An allosteric enzyme that follows the concerted model has an allosteric coefficient (T/R) of 300 in the absence of substrate.
Suppose that a mutation reversed the ratio.
Select the effects this mutation will have on the relationship between the rate of the reaction (V) and substrate
concentration, [S].
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The enzyme would likely follow Michaelis-Menten kinetics.
The plot of V versus [S] would be sigmoidal.
The enzyme would mostly be in the T form.
The plot of V versus [S] would be hyperbolic.
The enzyme would be more active.
Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also
known as ẞ-lactamase), an enzyme present in some penicillin-resistant
bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kDa.
The amount of penicillin hydrolyzed in 1 minute in a 10.0 mL. solution
containing 1.00 x 10 g of purified penicillinase was measured as a
function of the concentration of penicillin. Assume that the concentration of
penicillin does not change appreciably during the assay.
Plots of V versus [S] and 1/V versus 1/[S] for these data are shown.
Vo (* 10 M minute"¹)
7.0
6.0
5.0
4.0
3.0
20
1.0
0.0
о
10
20
30
1/Vo (* 10 M1 minute)
20
103
90
BO
70
50
[S] (* 100 M)
40
50
60
y=762x+1.46 × 10"
[Penicillin] (M)
Amount hydrolyzed (uM)
1
0.11
3
0.25
5
0.34
10
0.45
30
0.58
50
0.61
Chapter 28 Solutions
Study Guide With Student Solutions Manual And Problems Book For Garrett/grisham's Biochemistry, 6th
Ch. 28 - Semiconservative or Conservative DNA Replication...Ch. 28 - The Enzymatic Activities of DNA Polymerase I (a)...Ch. 28 - Multiple Replication Forks in E. coli I Assuming...Ch. 28 - Multiple Replication Forks in E. coli II On the...Ch. 28 - Molecules of DNA Polymerase III per Cell vs....Ch. 28 - Number of Okazaki Fragments in E. coli and Human...Ch. 28 - The Roles of Helicases and Gyrases How do DNA...Ch. 28 - Human Genome Replication Rate Assume DNA...Ch. 28 - Heteroduplex DNA Formation in Recombination From...Ch. 28 - Homologous Recombination, Heteroduplex DNA, and...
Ch. 28 - Prob. 11PCh. 28 - Prob. 12PCh. 28 - Chemical Mutagenesis of DNA Bases Show the...Ch. 28 - Prob. 14PCh. 28 - Recombination in Immunoglobulin Genes If...Ch. 28 - Helicase Unwinding of the E. coli Chromosome...Ch. 28 - Prob. 17PCh. 28 - Functional Consequences of Y-Family DNA Polymerase...Ch. 28 - Figure 28.11 depicts the eukaryotic cell cycle....Ch. 28 - Figure 28.41 gives some examples of recombination...Ch. 28 - Prob. 21PCh. 28 - Prob. 22P
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- Consider the reaction. kp S P kg What effects are produced by an enzyme on the general reaction? AG for the reaction increases. The rate constant for the reverse reaction (kr) increases. The reaction equilibrium is shifted toward the products. The concentration of the reactants is increased. The activation energy for the reaction is lowered. The formation of the transition state is promoted.arrow_forwardThe graph displays the activities of wild-type and several mutated forms of subtilisin on a logarithmic scale. The mutations are identified as: • The first letter is the one-letter abbreviation for the amino acid being altered. • The number identifies the position of the residue in the primary structure. ⚫ The second letter is the one-letter abbreviation for the amino acid replacing the original one. • Uncat. refers to the estimated rate for the uncatalyzed reaction. Log₁(S-1) Wild type S221A H64A -5 D32A S221A H64A D32A -10 Uncat. How would the activity of a reaction catalyzed by a version of subtilisin with all three residues in the catalytic triad mutated compare to the activity of the uncatalyzed reaction? It would have more activity, because the reaction catalyzed by the triple mutant is approximately three-fold faster than the uncatalyzed reaction. It would have less activity, because the reaction catalyzed by the triple mutant is approximately 1000-fold slower than the…arrow_forwardB Substrate Product AL Product Substrate Reaction progress- Reaction progress- omplete the passage describing the two reactions. In reaction A, the stability of the substrate is (AG) of the reaction is positive, Incorrect Answer greater than the stability of the product. The free-energy change Incorrect Answer so the reaction is considered In reaction B, the stability of the substrate is (AG) of the reaction is less than Incorrect Answer endergonic and Incorrect Answer not spontaneous. Incorrect Answer the stability of the product. The free-energy change negative, so the reaction is considered Incorrect Answer exergonic and spontaneous. Incorrect Answer Incorrect Answerarrow_forward
- Match the descriptions and compounds with the terms competitive, uncompetitive, and noncompetitive inhibition. Competitive inhibition Uncompetitive inhibition Noncompetitive inhibition Answer Bank inhibitor binds to the enzyme-substrate complex only Vmax remains the same but Ky increases inhibitor and substrate can bind simultaneously lowers Vmax and KM doxycycline sulfanilamide KM remains unchanged, but Vis lower prevents substrate from binding to the active site Rounduparrow_forwardDraw a pentapeptide made of the following amino acids: glycine, tyrosine, lysine, glutamate, and leucine at pH 1, pH 7, and pH 12. Draw the correct stereochemistry for each pentapeptide. Calculate the charge of the three compounds you've drawn and the PI.arrow_forward7. a. Complete the following redox reaction with the missing products NADH H b. Provide an arrow-pushing mechanism for the oxidation reaction. Use the explicit structure of NADH or NAD' as needed. For simplicity, use only the nicotinamide portion of the moleculearrow_forward
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