Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
7th Edition
ISBN: 9781464126116
Author: David L. Nelson, Michael M. Cox
Publisher: W. H. Freeman
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Chapter 28, Problem 3P

(a)

Summary Introduction

To explain:  The effect on gene expression in the lac operon of a mutation in lac operator that deletes most of O1

Introduction:

The lacIgene codes for the repressor molecule which negatively regulates the Lac operon, by binding to lacO1, O2 , and O3 operator. Binding of repressor molecule prevents the initiation of transcription, whereas binding of inducer to the repressor increase the efficiency of transcription.

(b)

Summary Introduction

To explain: The effect on gene expression in the lac operon of a mutation in lacIgene that inactivates the repressor

Introduction:

The lacIgene codes for the repressor molecule which negatively regulates the Lac operon, by binding to lacO1 , O2 , and O3 operator. Binding of repressor molecule prevents the initiation of transcription, whereas binding of inducer to the repressor increase the efficiency of transcription.

(c)

Summary Introduction

To determine: The effect on gene expression in the lac operon of a mutation in the promoter that alters the region around position 10

Introduction:

The lacIgene codes for the repressor molecule which negatively regulates the Lac operon, by binding to the lacO1 , O2 and O3 operator. Binding of repressor molecule prevents the initiation of transcription, whereas binding of inducer to the repressor increase the efficiency of transcription.

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