The reason why the complete and minimal nutrient replica plated. Introduction: Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Question

Want to see more full solutions like this?

Answer 1

Question

Chapter 28, Problem 1Q

Summary Introduction

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Expert Solution & Answer

Explanation of Solution

The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.

So, for the isolation purpose both media are important.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Students have asked these similar questions

What did the Cre-lox system used in the Kikuchi et al. 2010 heart regeneration experiment allow researchers to investigate? What was the purpose of the cmlc2 promoter? What is CreER and why was it used in this experiment? If constitutively active Cre was driven by the cmlc2 promoter, rather than an inducible CreER system, what color would you expect new cardiomyocytes in the regenerated area to be no matter what? Why?

What kind of organ size regulation is occurring when you graft multiple organs into a mouse and the graft weight stays the same?

What is the concept "calories consumed must equal calories burned" in regrads to nutrition?

Answer 2

Question

Chapter 28, Problem 1Q

Summary Introduction

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Expert Solution & Answer

Explanation of Solution

The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.

So, for the isolation purpose both media are important.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 3

Question

Chapter 28, Problem 1Q

Summary Introduction

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Expert Solution & Answer

Explanation of Solution

The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.

So, for the isolation purpose both media are important.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Question

Chapter 28, Problem 1Q

Summary Introduction

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Expert Solution & Answer

Explanation of Solution

The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.

So, for the isolation purpose both media are important.

Want to see more full solutions like this?

Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Chapter 28, Problem 1Q

Summary Introduction

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Expert Solution & Answer

Explanation of Solution

The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.

So, for the isolation purpose both media are important.

Answer 6

Chapter 28, Problem 1Q

Summary Introduction

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Expert Solution & Answer

Explanation of Solution

The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.

So, for the isolation purpose both media are important.

Answer 7

Chapter 28, Problem 1Q

Summary Introduction

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Answer 8

Expert Solution & Answer

Explanation of Solution

The anabolic mutants are not able to use thechemicals present in the glucose minimal salt media to grow. In case, this media lacks microbes that grew on the complete media, then it can be concluded the microbes that are not able to grow are the mutants or the auxotrophs. So, the colonies that appear in only the complete nutrient plate can be isolated for the isolation of mutants.

So, for the isolation purpose both media are important.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Ch. 28 - Prob. 1Q Ch. 28 - Prob. 2Q Ch. 28 - Prob. 1CT Ch. 28 - How would you identify the growth factor(s) needed...Ch. 28 - In one public health study, replica plating was...

The reason why the complete and minimal nutrient replica plated. Introduction: Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

To determine: The reason why the complete and minimal nutrient replica plated. Introduction: Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.

Explanation of Solution

Want to see more full solutions like this?

Chapter 28 Solutions

To determine:

The reason why the complete and minimal nutrient replica plated.

Introduction:

Replica plating is a technique, in which secondary plates having a minimal media along with other ingredients such as antibiotics are inoculated by transferring the colonies from primary plate.