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Interpretation:
The activated intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are to be stated. The leaving groups present in the enzymes are to be stated.
Concept introduction:
DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C). In the formation of recombinant DNA, the restriction enzymes are involved to cut the particular region in the DNA molecule.
Answer to Problem 1P
The activating intermediate present in the linkage of the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes, DNA polymerase I, DNA ligase and topoisomerase I are pyrophosphate, AMP and DNA- tyrosine residue respectively.
Explanation of Solution
It is given that the enzymes, DNA polymerase I, DNA ligase and topoisomerase I help in catalyzing the formation of phosphodiaster bonds.
The activated intermediate present in case of DNA polymerase I is deoxynucleotide triphosphate and the leaving group present in this enzyme is pyrophosphate.
The activated intermediate present in case of DNA ligase is DNA adenylate, in which AMP is attached to
The activated intermediate present in case of topoisomerase I is DNA-tyrosyl intermediate, in which
The activating intermediate present in the linkage of the given enzymes are deoxynucleotide triphosphate, DNA adenylate and DNA-tyrosyl intermediate respectively. The leaving groups present in the enzymes are pyrophosphate, AMP and DNA- tyrosine residue respectively.
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BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning