To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein. Introduction : The gal operon is present in yeast and regulates the galactose metabolism , by encoding the enzymes which are necessary for the process. Gal operon contain O E and O I operator site, present before the promoter.

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Answer 1

Question

Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.

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Fura-2 Fluorescence (Arbitrary Unit) 4500 4000 3500 3000 2500 2000 1500 1000 500 [Ca2+]=2970nM, 25°C [Ca2+] 2970nM, 4°C [Ca2+]=0.9nM, 25°C [Ca2+] = 0.9nM, 4°C 0 260 280 300 340 360 380 400 420 440 Wavelength (nm) ← < The figure on the LHS shows the excitation spectra of Fura-2 (Em = 510 nm) in 2 solutions with two different Ca2+ ion concentration as indicated. Except for temperature, the setting for excitation & signal acquisition was identical.< ப a) The unit in Y-axis is arbitrary (unspecified). Why? < < b) Compare & contrast the excitation wavelength of the Isosbestic Point of Fura-2 at 25 °C & 4 °C. Give a possible reason for the discrepancy. < c) The fluorescence intensity at 25 °C & 4 °C are different. Explain why with the concept of electronic configuration. <

draw in the structure of each amino acid (as L-amino acids) using the Fischer projection style. an example has been included. Draw the structure for glycine, alanine, valine, isoleucine, methionine, proline, phenylalanine, tryptophan, serine, threonine, asparagine, glutamine, lysine, arginine, aspartic acid, glutamic acid, histidine, tyrosine, cysteine

draw in the structure of each amino acid (as L-amino acids) using the Fischer projection style. an example has been included

Answer 2

Question

Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.

Expert Solution & Answer

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Check out a sample textbook solution

See solution

Answer 5

Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.

Answer 6

Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Answer 7

Chapter 28, Problem 11P

Summary Introduction

To draw: The diagram of different functional domains that would be found in the Gal4 protein and in the engineered protein.

Introduction:

The gal operon is present in yeast and regulates the galactose metabolism, by encoding the enzymes which are necessary for the process. Gal operon contain O_E and O_I operator site, present before the promoter.

Answer 8

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Answer 9

Summary Introduction

To determine: The reason that engineered protein no longer regulates transcription of GAL genes.

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding protein, and facilitates the activator proteins to initiate the transcription.

Answer 10

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.

Answer 11

Summary Introduction

To determine: The mechanism by which DNA binding site by this chimeric protein to make it functional in activating transcription of GAL genes

Introduction:

DNA sequence contain DNA binding domain for binding of proteins involve in gene regulation. RNA polymerase binds to DNA binding domain, and facilitates the activator proteins to initiate the transcription.

Answer 12

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Answer 15

Ch. 28 - Prob. 1P Ch. 28 - Prob. 2P Ch. 28 - Prob. 3P Ch. 28 - Prob. 4P Ch. 28 - Prob. 5P Ch. 28 - Prob. 6P Ch. 28 - Prob. 7P Ch. 28 - Prob. 8P Ch. 28 - Prob. 9P Ch. 28 - Prob. 10P

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