Campbell Biology: Custom Edition
18th Edition
ISBN: 9781323717271
Author: Urry, Cain, Wasserman, Minorsky, Reece
Publisher: PEARSON C
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Textbook Question
Chapter 27.3, Problem 2CC
A bacterium requires only the amino acid methionine as an organic nutrient and lives in lightless caves. What mode of nutrition does it employ? Explain.
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True or Falso
a) ____Ti plasmid cloning requires the use of the Agrobacterium tumefaciens to insert a recombinant gene into a target plant.
b) ____Vitamin A deficiency is a serious disease that can cause blindness and death in children, but genetic engineering has created a novel food product called golden rice that is fortified with Vitamin A and is freely available for use in rice-dependent countries order to combat this deficiency.
Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description
True or False?
a.____If a restriction enzyme recognizes the restriction site, 5’AACGTT3’, and the enzyme cuts between the second A and the C, this will produce a “sticky end,” which is useful for cloning into a plasmid vector.
b.____The DNA molecules will travel through the agar toward the negative end of the gel rig (black wires) during electrophoresis.
c.____CRISPR was one of the first methods discovered in the 1970s for reliably amplifying gene products so that they can be sequenced and studied.
Chapter 27 Solutions
Campbell Biology: Custom Edition
Ch. 27.1 - Describe two adaptations that enable prokaryotes...Ch. 27.1 - Contrast the cellular and DNA structures of...Ch. 27.1 - Prob. 3CCCh. 27.2 - Although rare on a per gene basis, new mutations...Ch. 27.2 - Distinguish between the three mechanisms by which...Ch. 27.2 - In a rapidly changing environment, which bacterial...Ch. 27.2 - WHAT IF? If a nonpathogenic bacterium were to...Ch. 27.3 - Distinguish between the four major modes of...Ch. 27.3 - A bacterium requires only the amino acid...Ch. 27.3 - WHAT IF? Describe what you might eat for a...
Ch. 27.4 - Explain how molecular systematics and metagenomics...Ch. 27.4 - WHAT IF What would the discovery of a bacterial...Ch. 27.5 - Explain how prokaryotes, though small, can be...Ch. 27.5 - MAKE CONNECTIONS Review Figure 10.6. Then...Ch. 27.6 - Identify at least two ways that prokaryotes have...Ch. 27.6 - Prob. 2CCCh. 27.6 - Prob. 3CCCh. 27 - Describe features of prokaryotes that enable them...Ch. 27 - Mutations are rare and prokaryotes reproduce...Ch. 27 - Describe the range of prokaryotic metabolic...Ch. 27 - How have molecular data informed prokaryotic...Ch. 27 - In what ways are prokaryotes key to the survivaI...Ch. 27 - Prob. 27.6CRCh. 27 - Genetic variation in bacterial populations cannot...Ch. 27 - Photoautotrophs use (A) light as an energy source...Ch. 27 - Which of the following statements is not true? (A)...Ch. 27 - Prob. 4TYUCh. 27 - Prob. 5TYUCh. 27 - Prob. 6TYUCh. 27 - Prob. 7TYUCh. 27 - SCIENTIFIC INQUIRY INTERPRET THE DATA The...Ch. 27 - WRITE ABOUT A THEME: ENERGY In a short essay...Ch. 27 - SYNTHESIZE YOUR KNOWLEDGE Explain how the small...
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