Chapter 27.3, Problem 27.3CIAP

Interpretation Introduction

Interpretation:

Why Taq polymerase used in PCR instead of the DNA polymerase found in humans has to be explained.

Concept Introduction:

Polymerase chain reaction (PCR):

Polymerase chain reaction (PCR) is a technique which is used for amplification of DNA strand by thermos aquaticus polymerase (Taq polymerase).

Taq polymerase is an enzyme which is used in the PCR reaction because it can withstand very high temperatures because PCR reaction involves different temperatures; denaturation temperature (${\text{95}}^0\text{C}$), annealing temperature (${\text{55}}^0\text{C}$) and extension temperature (${72}^0\text{C}$).